Abstract | OBJECTIVE: APPROACH AND RESULTS: Healthy overweight or obese human subjects underwent adipose-tissue biopsies and quantification of insulin-mediated glucose disposal by the modified insulin suppression test. T-cell subsets were quantified by flow cytometry in visceral (VAT) and subcutaneous adipose tissue (SAT). Results showed that CD4 and CD8 T cells infiltrate both depots, with proinflammatory T-helper (Th)-1, Th17, and CD8 T cells, significantly more frequent in VAT as compared with SAT. T-cell profiles in SAT and VAT correlated significantly with one another and with peripheral blood. Th1 frequency in SAT and VAT correlated directly, whereas Th2 frequency in VAT correlated inversely, with plasma high-sensitivity C-reactive protein concentrations. Th2 in both depots and peripheral blood was inversely associated with systemic insulin resistance. Furthermore, Th1 in SAT correlated with plasma interleukin-6. Relative expression of associated cytokines, measured by real-time polymerase chain reaction, reflected flow cytometry results. Most notably, adipose tissue expression of anti-inflammatory interleukin-10 was inversely associated with insulin resistance. CONCLUSIONS:
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Authors | Tracey McLaughlin, Li-Fen Liu, Cindy Lamendola, Lei Shen, John Morton, Homero Rivas, Daniel Winer, Lorna Tolentino, Okmi Choi, Hong Zhang, Melissa Hui Yen Chng, Edgar Engleman |
Journal | Arteriosclerosis, thrombosis, and vascular biology
(Arterioscler Thromb Vasc Biol)
Vol. 34
Issue 12
Pg. 2637-43
(Dec 2014)
ISSN: 1524-4636 [Electronic] United States |
PMID | 25341798
(Publication Type: Clinical Trial, Journal Article, Research Support, N.I.H., Extramural)
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Copyright | © 2014 American Heart Association, Inc. |
Chemical References |
- Cytokines
- Inflammation Mediators
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Topics |
- Adipose Tissue
(immunology, pathology)
- Adult
- Aged
- Animals
- Cytokines
(blood, genetics)
- Female
- Humans
- Inflammation
(genetics, immunology, pathology)
- Inflammation Mediators
(blood)
- Insulin Resistance
(genetics, immunology)
- Intra-Abdominal Fat
(immunology, pathology)
- Male
- Mice
- Middle Aged
- Obesity
(genetics, immunology, pathology)
- Overweight
(genetics, immunology, pathology)
- Subcutaneous Fat
(immunology, pathology)
- T-Lymphocyte Subsets
(immunology, pathology)
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