Abstract | OBJECTIVE: BACKGROUND:
Ischemia/reperfusion resulting from S/R contributes to multiple organ dysfunction in trauma patients. We hypothesized that RIC before shock (remote ischemic preconditioning), during shock (remote ischemic "PER"conditioning), or during resuscitation (remote ischemic "POST"conditioning) could confer organ protection. We also tested the effect of ischemic conditioned plasma on neutrophil migration in vivo using transgenic zebrafish models. METHODS: RESULTS: In mice subjected to S/R, remote ischemic preconditioning, remote ischemic "PER"conditioning, and remote ischemic "POST"conditioning each significantly reduced serum alanine aminotransferase and liver mRNA expression of tumor necrosis factor-alpha and interleukin 1β and improved liver histology compared with control S/R mice. Lung injury and inflammation were also significantly reduced in mice treated with remote ischemic preconditioning. Zebrafish injected with plasma or dialyzed plasma (fraction >14 kDa) from ischemic conditioned mice had reduced neutrophil migration toward sites of injury compared with zebrafish injected with control plasma. CONCLUSIONS: RIC protects against S/R-induced organ injury, in part, through a humoral factor(s), which alters neutrophil function. The beneficial effects of RIC, performed during the S/R phase of care, suggest a role for its application early in the posttrauma period.
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Authors | Chung Ho Leung, Christopher A Caldarone, Feng Wang, Seetha Venkateswaran, Menachem Ailenberg, Brian Vadasz, Xiao-Yan Wen, Ori D Rotstein |
Journal | Annals of surgery
(Ann Surg)
Vol. 261
Issue 6
Pg. 1215-25
(Jun 2015)
ISSN: 1528-1140 [Electronic] United States |
PMID | 25185480
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers
- Interleukin-1beta
- Tumor Necrosis Factor-alpha
- Alanine Transaminase
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Topics |
- Alanine Transaminase
(blood)
- Animals
- Animals, Genetically Modified
- Biomarkers
(blood)
- Chemotaxis, Leukocyte
- Disease Models, Animal
- Interleukin-1beta
(blood)
- Ischemic Preconditioning
- Liver Diseases
(blood, etiology, pathology)
- Lung Injury
(blood, etiology, pathology)
- Male
- Mice
- Mice, Inbred C57BL
- Neutrophils
(immunology)
- Plasma
(immunology)
- Reperfusion Injury
(blood, etiology, immunology, prevention & control)
- Shock, Hemorrhagic
(blood, complications, immunology, therapy)
- Systemic Inflammatory Response Syndrome
(immunology, prevention & control)
- Time Factors
- Treatment Outcome
- Tumor Necrosis Factor-alpha
(blood)
- Zebrafish
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