Interleukin-25 Enhances Allergic Inflammation through p38MAPK and NF-κB Pathways in Mouse Models of Allergic Rhinitis.

Abstract

Interleukin (IL)-25, a cytokine of IL-17 family, can activate p38 Mitogen-Activated Protein kinases(MAPK) and Nuclear Factor(NF)-κB pathways to propagate Th2 responses. The allergic rhinitis mouse model was established by stimulating BALB/c mouse with ovalbumin (OVA). Then we detected expression of IL-25 and downstream p38MAPK and NF-κB. The expression of IL-25, p38MAPK and NF-κB were detected in the OVA-induced allergic rhinitis mouse model. The allergic parameters, such as allergic symptoms, serum OVA-specific immunoglobulin E (IgE) levels and eosinophil infiltration in the nasal mucosa were compared between OVA group and control group. OVA-induced mice displayed significantly higher allergic responses compared with the saline control group. OVA induced mice demonstrated more allergic symptoms, higher serum OVA-specific IgE levels and eosinophil infiltrations. The increased expression of IL-25, p38MAPK and NF-κB immunoreactivity were detected in epidermal cells in the OVA group. The mRNA measurement of IL-25, p38MAPK and NF-κB showed the same result. IL-25 enhances the OVA-induced allergic rhinitis by activating p38MAPK and NF-κB pathways.

• Authors: Zhipeng Li, Hui Wang, Lisi Liu
• Journal: Iranian journal of allergy, asthma, and immunology (Iran J Allergy Asthma Immunol) Vol. 13 Issue 6 Pg. 412-9 (Dec 2014) ISSN: 1735-1502 [Print] Iran
• PMID: 25148799 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Interleukins
• Mydgf protein, mouse
• NF-kappa B
• Immunoglobulin E
• Ovalbumin
• p38 Mitogen-Activated Protein Kinases

Topics

• Animals
• Disease Models, Animal
• Enzyme-Linked Immunosorbent Assay
• Female
• Immunoglobulin E (blood)
• Interleukins (analysis, genetics, physiology)
• Male
• Mice
• Mice, Inbred BALB C
• NF-kappa B (analysis, genetics, physiology)
• Ovalbumin (immunology)
• Rhinitis, Allergic (etiology)
• Signal Transduction (physiology)
• p38 Mitogen-Activated Protein Kinases (analysis, genetics, physiology)