PF2401-SF, standardized fraction of Salvia miltiorrhiza shows anti-inflammatory activity in macrophages and acute arthritis in vivo.

Abstract

Standardization of processing methods for herbs is as important as authentication to maintain their quality and ensure their safe use. We had previously prepared a standardized and purified Salvia miltiorrhiza Bunge extract, PF2401-SF, and showed that it protects against liver injury in vivo, at a greater potency than an ethanol extract. PF2401-SF was enriched with tanshinone I (11.5%), tanshinone IIA (41.0%), and cryptotanshinone (19.1%). In this study, we investigated potential anti-inflammatory effects of PF2401-SF in vitro and in vivo. We demonstrated that PF2401-SF shows anti-inflammatory potency on lipopolysaccharide (LPS)-induced nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression in RAW 264.7 cells. A mechanistic study indicated that PF2401-SF induced heme oxygenase (HO)-1 expression through extracellular signal-regulated kinases (ERK1/2) phosphorylation. Moreover, we also evaluated that PF2401-SF significantly reduced inflammation on carrageenan- or dextran-induced acute arthritis in rats. Our results suggest that PF2401-SF may be a potential candidate for the treatment of various inflammatory diseases.

• Authors: Wen-Yi Jiang, Byeong-Ho Jeon, Youn-Chul Kim, Sung Hee Lee, Dong Hwan Sohn, Geom Seog Seo
• Journal: International immunopharmacology (Int Immunopharmacol) Vol. 16 Issue 2 Pg. 160-4 (Jun 2013) ISSN: 1878-1705 [Electronic] Netherlands
• PMID: 23583334 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier B.V. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Dextrans
• Lipopolysaccharides
• Membrane Proteins
• PF2401-SF
• Plant Extracts
• Nitric Oxide
• Carrageenan
• Nitric Oxide Synthase Type II
• Nos2 protein, mouse
• Heme Oxygenase-1
• Hmox1 protein, mouse
• Extracellular Signal-Regulated MAP Kinases

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology, therapeutic use)
• Arthritis (chemically induced, drug therapy)
• Carrageenan
• Cell Line
• Dextrans
• Edema (chemically induced, drug therapy)
• Extracellular Signal-Regulated MAP Kinases (metabolism)
• Heme Oxygenase-1 (metabolism)
• Lipopolysaccharides
• Macrophages
• Male
• Membrane Proteins (metabolism)
• Mice
• Nitric Oxide (metabolism)
• Nitric Oxide Synthase Type II (metabolism)
• Plant Extracts (pharmacology, therapeutic use)
• Plant Roots
• Rats
• Rats, Wistar
• Salvia miltiorrhiza