Abstract | BACKGROUND: METHODS: RESULTS: Mean clinical sickness score, macroscopic gut assessment score, and intestinal injury score were significantly improved, whereas mean apoptosis score and caspase-3 levels were significantly reduced in pups in the NEC + CDP-choline group compared with the NEC group. Tissue proinflammatory cytokine ( interleukin-1β, interleukin-6, and tumor necrosis factor-α) levels as well as tissue malondialdehyde content and myeloperoxidase activities were reduced, whereas glutathione peroxidase and superoxide dismutase activities were preserved in the NEC + CDP-choline group. In addition, NEC damage reduced intestinal tissue membrane phospholipids, whereas CDP-choline significantly enhanced total phospholipid and phosphatidylcholine levels. Long-term follow-up in additional experiments revealed increased body weight, decreased clinical sickness scores, and enhanced survival in CDP-choline-receiving versus saline-receiving pups with NEC lesions. CONCLUSIONS: Our study reports, for the first time, beneficial effects of CDP-choline treatment on intestinal injury in a neonatal rat model of NEC. Our data suggest that CDP-choline may be used as an effective therapeutic agent to prevent NEC.
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Authors | Merih Cetinkaya, Mehmet Cansev, Ferhat Cekmez, Cuneyt Tayman, Fuat Emre Canpolat, Ilker M Kafa, Sema Uysal, Turan Tunc, S Umit Sarici |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 183
Issue 1
Pg. 119-28
(Jul 2013)
ISSN: 1095-8673 [Electronic] United States |
PMID | 23228325
(Publication Type: Journal Article)
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Copyright | Copyright © 2013 Elsevier Inc. All rights reserved. |
Chemical References |
- Cytokines
- Nootropic Agents
- Cytidine Diphosphate Choline
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Topics |
- Animals
- Animals, Newborn
- Apoptosis
(drug effects)
- Cytidine Diphosphate Choline
(pharmacology, therapeutic use)
- Cytokines
(metabolism)
- Disease Models, Animal
- Drug Evaluation, Preclinical
- Enterocolitis, Necrotizing
(enzymology, pathology, prevention & control)
- Intestines
(enzymology, pathology)
- Nootropic Agents
(pharmacology, therapeutic use)
- Rats
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