Effects of astragaloside IV on eosinophil activation induced by house dust mite allergen.

Abstract

Astragaloside IV (AS-IV) has been noted for its reduction of eosinophilic airway inflammation in a murine model of chronic asthma. To gain a better understanding of the mechanisms involved in this anti-inflammatory phenomenon, the effect of AS-IV on human blood eosinophils was studied in vitro. Eosinophils were isolated from the blood of patients with mild atopic asthma, preincubated with AS-IV for 1 h and stimulated in the presence or absence of the house dust mite allergen Dermatophagoides pteronyssinus (Der p) 1 for 4 h. The survival of the eosinophils at 48 h was investigated using trypan blue and the surface expression of CC chemokine receptor 3 (CCR3) and intercellular adhesion molecule-1 (ICAM-1) by the eosinophils was analyzed using flow cytometry. The secretion of cytokines in the supernatants and the chemotaxis of the eosinophils were measured by ELISA and the transwell system, respectively. Der p 1 was found to prolong the survival of the eosinophils. Similarly, the expression of CCR3 and ICAM-1, secretion of interleukin (IL)-1β, IL-5, tumor necrosis factor (TNF)-α and the granulocyte macrophage colony stimulating factor (GM-CSF) and transmigration of the eosinophils were increased in the presence of Der p 1. However, these inductive effects on the eosinophils were significantly inhibited by AS-IV (50 µg/ml). These findings suggest that AS-IV modulates eosinophil activation and trafficking in response to Der p 1 and may therefore be a useful therapeutic option in eosinophilic asthma.

• Authors: Xiaoyan Gu, Desheng Jiang, Yuwei Wang, Qiang Du, Jiankang Cai
• Journal: Molecular medicine reports (Mol Med Rep) Vol. 6 Issue 1 Pg. 115-20 (07 2012) ISSN: 1791-3004 [Electronic] Greece
• PMID: 22505212 (Publication Type: Journal Article)

Chemical References

• Allergens
• Anti-Inflammatory Agents
• Antigens, Dermatophagoides
• Arthropod Proteins
• Cytokines
• Drugs, Chinese Herbal
• Receptors, CCR3
• Saponins
• Triterpenes
• Intercellular Adhesion Molecule-1
• astragaloside A
• Cysteine Endopeptidases
• Dermatophagoides pteronyssinus antigen p 1

Topics

• Allergens (immunology)
• Anti-Inflammatory Agents (chemistry, pharmacology)
• Antigens, Dermatophagoides (immunology)
• Arthropod Proteins (immunology)
• Cell Survival (drug effects, immunology)
• Cells, Cultured
• Cysteine Endopeptidases (immunology)
• Cytokines (biosynthesis)
• Drugs, Chinese Herbal (chemistry, pharmacology)
• Eosinophils (drug effects, immunology, metabolism)
• Humans
• Intercellular Adhesion Molecule-1 (metabolism)
• Receptors, CCR3 (metabolism)
• Saponins (chemistry, pharmacology)
• Time Factors
• Transendothelial and Transepithelial Migration (drug effects, immunology)
• Triterpenes (chemistry, pharmacology)