Rapid and reliable quantitation of amino acids and myo-inositol in mouse brain by high performance liquid chromatography and tandem mass spectrometry.

Abstract

Amino acids and myo-inositol have long been proposed as putative biomarkers for neurodegenerative diseases. Accurate measures and stability have precluded their selective use. To this end, a sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method based on multiple reaction monitoring was developed to simultaneously quantify glutamine, glutamate, γ-aminobutyric acid (GABA), aspartic acid, N-acetyl aspartic acid, taurine, choline, creatine, phosphocholine and myo-inositol in mouse brain by methanol extractions. Chromatography was performed using a hydrophilic interaction chromatography silica column within in a total run time of 15 min. The validated method is selective, sensitive, accurate, and precise. The method has a limit of quantification ranging from 2.5 to 20 ng/ml for a range of analytes and a dynamic range from 2.5-20 to 500-4000 ng/ml. This LC-MS/MS method was validated for biomarker discovery in models of human neurological disorders.

• Authors: Sai P Bathena, Jiangeng Huang, Adrian A Epstein, Howard E Gendelman, Michael D Boska, Yazen Alnouti
• Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (J Chromatogr B Analyt Technol Biomed Life Sci) Vol. 893-894 Pg. 15-20 (Apr 15 2012) ISSN: 1873-376X [Electronic] Netherlands
• PMID: 22425384 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: Copyright © 2012 Elsevier B.V. All rights reserved.

Chemical References

• Amino Acids
• Inositol

Topics

• Amino Acids (analysis)
• Animals
• Brain Chemistry
• Chromatography, High Pressure Liquid (methods)
• Hydrophobic and Hydrophilic Interactions
• Inositol (analysis)
• Mice
• Reproducibility of Results
• Sensitivity and Specificity
• Tandem Mass Spectrometry (methods)