Abstract | BACKGROUND: METHODS: RESULTS:
IL-22 was detected at 24 hr but not 6 hr of liver IRI. The expression of IL-22R1 was increased by 6 hr of reperfusion in WT but not type 1 interferon receptor KO mice that were protected from IRI. Treatment of WT mice with recombinant IL-22 decreased serum aspartate aminotransferase levels, ameliorated cardinal histological features of IR damage (Suzuki's score) and diminished leukocyte sequestration, along with the expression of IL-22R1 and pro-inflammatory cytokines. IL-22 antibody did not appreciably affect IRI but increased IL-22R1 transcription in the liver. Administration of IL-22 protein exerted hepatoprotection by STAT3 activation. CONCLUSIONS: This is the first report investigating immune modulation by T-cell-derived IL-22 in liver injury caused by warm ischemia and reperfusion. Treatment with IL-22 protein may represent a novel therapeutic strategy to prevent liver IRI in transplant recipients.
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Authors | Paul J Chestovich, Yoichiro Uchida, William Chang, Mark Ajalat, Charles Lassman, Robert Sabat, Ronald W Busuttil, Jerzy W Kupiec-Weglinski |
Journal | Transplantation
(Transplantation)
Vol. 93
Issue 5
Pg. 485-92
(Mar 15 2012)
ISSN: 1534-6080 [Electronic] United States |
PMID | 22262131
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Neutralizing
- Ifnar2 protein, mouse
- Inflammation Mediators
- Interleukins
- Receptors, Interleukin
- Recombinant Proteins
- STAT3 Transcription Factor
- Stat3 protein, mouse
- interleukin-22 receptor
- Receptor, Interferon alpha-beta
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Topics |
- Animals
- Antibodies, Neutralizing
(administration & dosage)
- Disease Models, Animal
- Inflammation Mediators
(metabolism)
- Interleukins
(administration & dosage, antagonists & inhibitors, metabolism)
- Liver
(blood supply, immunology, pathology)
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Receptor, Interferon alpha-beta
(deficiency, genetics)
- Receptors, Interleukin
(metabolism)
- Recombinant Proteins
(metabolism)
- Reperfusion Injury
(immunology, pathology, prevention & control)
- STAT3 Transcription Factor
(metabolism)
- Time Factors
- Interleukin-22
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