Abstract | OBJECTIVE: RESEARCH DESIGN AND METHODS:
Insulin sensitivity was analyzed through euglycemic-hyperinsulinemic clamp, and subclinical atherosclerosis was analyzed through intimal medial thickness. Monocytes were isolated through magnetic cell sorting, and mRNA and proteins were extracted and analyzed by quantitative PCR and pathscan enzyme-linked immunosorbent assays, respectively. RESULTS: In monocyte cells from human subjects with increased risk for diabetes and atherosclerosis, we found that gene expression, protein levels, and tyrosine phosphorylation of IRS2, but not InsR or IRS1, were decreased. TIMP3 was also reduced, along with insulin resistance, resulting in increased ectodomain shedding activity of the metalloprotease ADAM17. CONCLUSIONS:
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Authors | Marina Cardellini, Rossella Menghini, Alessio Luzi, Francesca Davato, Iris Cardolini, Rossella D'Alfonso, Paolo Gentileschi, Stefano Rizza, Maria Adelaide Marini, Ottavia Porzio, Davide Lauro, Paolo Sbraccia, Renato Lauro, Massimo Federici |
Journal | Diabetes
(Diabetes)
Vol. 60
Issue 12
Pg. 3265-70
(Dec 2011)
ISSN: 1939-327X [Electronic] United States |
PMID | 21984580
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- IRS2 protein, human
- Insulin
- Insulin Receptor Substrate Proteins
- Tissue Inhibitor of Metalloproteinase-3
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Topics |
- Adult
- Atherosclerosis
(metabolism)
- Carotid Intima-Media Thickness
- Diabetes Mellitus, Type 2
- Enzyme-Linked Immunosorbent Assay
- Female
- Glucose Clamp Technique
- Humans
- Insulin
(metabolism)
- Insulin Receptor Substrate Proteins
(genetics, metabolism)
- Insulin Resistance
(genetics, physiology)
- Male
- Middle Aged
- Monocytes
(metabolism)
- Polymerase Chain Reaction
- Tissue Inhibitor of Metalloproteinase-3
(genetics, metabolism)
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