Abstract |
The high-affinity receptor for IgE (FcɛRI)-mediated activation of mast cells plays an important role in allergic diseases such as asthma, allergic rhinitis and atopic dermatitis. Emodin, a naturally occurring anthraquinone derivative in oriental herbal medicines, has several beneficial pharmacologic effects, such as anti- cancer and anti-diabetic activities. However, the anti-allergic effect of emodin has not yet been investigated. To assess the anti-allergic activity of emodin, in vivo passive anaphylaxis animal model and in vitro mouse bone marrow-derived mast cells were used to investigate the mechanism of its action on mast cells. Our results showed that emodin inhibited degranulation, generation of eicosanoids ( prostaglandin D(2) and leukotriene C(4)), and secretion of cytokines (TNF-α and IL-6) in a dose-dependent manner in IgE/Ag-stimulated mast cells. Biochemical analysis of the FcɛRI-mediated signaling pathways demonstrated that emodin inhibited the phosphorylation of Syk and multiple downstream signaling processes including mobilization of intracellular Ca(2+) and activation of the mitogen-activated protein kinase, phosphatidylinositol 3-kinase, and NF-κB pathways. When administered orally, emodin attenuated the mast cell-dependent passive anaphylactic reaction in IgE-sensitized mice. Thus, emodin inhibits mast cell activation and thereby the anaphylactic reaction through suppression of the receptor-proximal Syk-dependent signaling pathways. Therefore, emodin might provide a basis for development of a novel anti-allergic drug.
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Authors | Yue Lu, Ju Hye Yang, Xian Li, Kyoung Hwangbo, Seung-Lark Hwang, Yoshitaka Taketomi, Makoto Murakami, Young-Chae Chang, Cheorl-Ho Kim, Jong-Keun Son, Hyeun Wook Chang |
Journal | Biochemical pharmacology
(Biochem Pharmacol)
Vol. 82
Issue 11
Pg. 1700-8
(Dec 01 2011)
ISSN: 1873-2968 [Electronic] England |
PMID | 21907188
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Crown Copyright © 2011. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Anti-Allergic Agents
- Interleukin-6
- Intracellular Signaling Peptides and Proteins
- Tumor Necrosis Factor-alpha
- Leukotriene C4
- Immunoglobulin E
- Protein-Tyrosine Kinases
- Syk Kinase
- Syk protein, mouse
- Emodin
- Prostaglandin D2
- Calcium
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Topics |
- Anaphylaxis
(immunology, metabolism, prevention & control)
- Animals
- Anti-Allergic Agents
(pharmacology, therapeutic use)
- Calcium
(metabolism)
- Cell Degranulation
(drug effects)
- Cells, Cultured
- Emodin
(pharmacology, therapeutic use)
- Enzyme Activation
- Immunoglobulin E
(immunology)
- Interleukin-6
(biosynthesis)
- Intracellular Signaling Peptides and Proteins
(metabolism)
- Leukotriene C4
(biosynthesis)
- Male
- Mast Cells
(drug effects, immunology, physiology)
- Mice
- Mice, Inbred BALB C
- Passive Cutaneous Anaphylaxis
- Prostaglandin D2
(biosynthesis)
- Protein-Tyrosine Kinases
(metabolism)
- Syk Kinase
- Tumor Necrosis Factor-alpha
(biosynthesis)
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