Membrane-rich milk fat diet provides protection against gastrointestinal leakiness in mice treated with lipopolysaccharide.

Abstract

Milk fat globule membrane is a protein-lipid complex that may strengthen the gut barrier. The main objective of this study was to assess the ability of a membrane-rich milk fat diet to promote the integrity of the gut barrier and to decrease systemic inflammation in lipopolysaccharide (LPS)-challenged mice. Animals were randomly assigned to one of 2 American Institute of Nutrition (AIN)-76A formulations differing only in fat source: control diet (corn oil) and milk fat diet (anhydrous milk fat with 10% milk fat globule membrane). Each diet contained 12% calories from fat. Mice were fed diets for 5 wk, then injected with vehicle or LPS (10mg/kg of BW) and gavaged with dextran-fluorescein to assess gut barrier integrity. Serum was assayed for fluorescence 24h after gavage, and 16 serum cytokines were measured to assess the inflammatory response. Gut permeability was 1.8-fold higher in LPS-challenged mice fed the control diet compared with the milk fat diet. Furthermore, mice fed the milk fat diet and injected with LPS had lower serum levels of IL-6, IL-10, IL-17, monocyte chemotactic protein (MCP)-1, interferon (IFN)-γ, tumor necrosis factor (TNF)-α, and IL-3 compared with LPS-injected mice fed the control diet. The results indicate that the membrane-rich milk fat diet decreases the inflammatory response to a systemic LPS challenge compared with corn oil, and the effect coincides with decreased gut permeability.

• Authors: D R Snow, R E Ward, A Olsen, R Jimenez-Flores, K J Hintze
• Journal: Journal of dairy science (J Dairy Sci) Vol. 94 Issue 5 Pg. 2201-12 (May 2011) ISSN: 1525-3198 [Electronic] United States
• PMID: 21524510 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

Chemical References

• Cytokines
• Glycolipids
• Glycoproteins
• Lipopolysaccharides
• milk fat globule
• Corn Oil

Topics

• Animal Nutritional Physiological Phenomena
• Animals
• Corn Oil (pharmacology)
• Cytokines (blood)
• Diet
• Gastrointestinal Diseases (prevention & control)
• Glycolipids (pharmacology, therapeutic use)
• Glycoproteins (pharmacology, therapeutic use)
• Inflammation (chemically induced, prevention & control)
• Intestinal Mucosa (drug effects, metabolism)
• Lipid Droplets
• Lipopolysaccharides (pharmacology)
• Male
• Mice
• Mice, Inbred BALB C
• Permeability (drug effects)
• Random Allocation