Methylation pattern of IFN-γ and IL-10 genes in periodontal tissues.

Abstract

UNLABELLED:

DNA methylation is characterized by the addition of methyl groups in cytosines within CpG islands. Unmethylated CpGs are related to transcriptionally active structure, whereas methylated CpG recruits methyl-binding proteins that promote chromatin compaction. DNA methylation can influence the expression of cytokines and affect the development of periodontal disease.

OBJECTIVES:

The purpose of the present study was to evaluate the methylation status of the interferon gamma (IFN-γ) and interleukin-10 (IL-10) genes in periodontal tissues.

DESIGN:

Methylation-specific polymerase chain reaction (MSP) and DNA sequencing analysis were used to verify the DNA methylation status of the IFN-γ and IL-10 genes, respectively, in samples from subjects without periodontitis and individuals with chronic periodontitis. Histological sections stained by hematoxylin-eosin were used for histopathological evaluation of samples.

RESULTS:

The methylation status of the IFN-γ and IL-10 genes was similar among the groups. Most of the samples were positive for IFN-γ methylation. Only 11% of the periodontitis group showed unmethylated DNA. Considering the IL-10 gene, no unmethylated sample was observed. The profile of total or partial methylation was detected in CpGs evaluated.

CONCLUSIONS:

The results showed evidence that methylation of IFN-γ and IL-10 genes is a usual feature on periodontal tissues. Further studies are needed to determine the functional relevance of these alterations.

• Authors: Michelle Beatriz Viana, Fabiano Pereira Cardoso, Marina Gonçalves Diniz, Fernando Oliveira Costa, José Eustáquio da Costa, Ricardo Santiago Gomez, Paula Rocha Moreira
• Journal: Immunobiology (Immunobiology) Vol. 216 Issue 8 Pg. 936-41 (Aug 2011) ISSN: 1878-3279 [Electronic] Netherlands
• PMID: 21281983 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier GmbH. All rights reserved.

Chemical References

• Chromatin
• Interleukin-10
• Interferon-gamma
• Cytosine

Topics

• Base Sequence
• Case-Control Studies
• Chromatin (genetics, immunology, metabolism)
• Chronic Periodontitis (genetics, immunology, metabolism)
• CpG Islands (immunology)
• Cytosine (metabolism)
• DNA Methylation (immunology)
• Humans
• Interferon-gamma (genetics, metabolism)
• Interleukin-10 (genetics, metabolism)
• Molecular Sequence Data
• Polymerase Chain Reaction
• Promoter Regions, Genetic (immunology)
• Sequence Analysis, DNA
• Transcription, Genetic (genetics, immunology)