Non-invasive limb ischemic pre-conditioning reduces oxidative stress and attenuates myocardium ischemia-reperfusion injury in diabetic rats.

Abstract

This study was to explore whether repeated non-invasive limb ischemic pre-conditioning (NLIP) can confer an equivalent cardioprotection against myocardial ischemia-reperfusion (I/R) injury in acute diabetic rats to the extent of conventional myocardial ischemic pre-conditioning (MIP) and whether or not the delayed protection of NLIP is mediated by reducing myocardial oxidative stress after ischemia-reperfusion. Streptozotocin-induced diabetic rats were randomized to four groups: Sham group, the I/R group, the MIP group and the NLIP group. Compared with the I/R group, both the NLIP and MIP groups showed an amelioration of ventricular arrhythmia, reduced myocardial infarct size, increased activities of total superoxide dismutase (SOD), manganese-SOD and glutathione peroxidase, increased expression of manganese-SOD mRNA and decreased xanthine oxidase activity and malondialdehyde concentration (All p < 0.05 vs I/R group). It is concluded that non-invasive limb ischemic pre-conditioning reduces oxidative stress and attenuates myocardium ischemia-reperfusion injury in diabetic rats.

• Authors: Xue-Hui Zhu, Heng-Jie Yuan, Yan-Na Wu, Yi Kang, Jian-Jie Jiao, Wei-Zhen Gao, Yan-Xia Liu, Jian-Shi Lou, Zhengyuan Xia
• Journal: Free radical research (Free Radic Res) Vol. 45 Issue 2 Pg. 201-10 (Feb 2011) ISSN: 1029-2470 [Electronic] England
• PMID: 20942563 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Malondialdehyde
• Streptozocin
• Glutathione Peroxidase
• Superoxide Dismutase
• Xanthine Oxidase

Topics

• Animals
• Arrhythmias, Cardiac (metabolism, physiopathology)
• Blood Pressure
• Diabetes Mellitus, Experimental (metabolism, physiopathology)
• Extremities (blood supply, physiopathology)
• Gene Expression
• Glutathione Peroxidase (metabolism)
• Hemodynamics
• Ischemic Preconditioning (methods)
• Male
• Malondialdehyde (metabolism)
• Models, Animal
• Myocardial Infarction (metabolism, physiopathology)
• Myocardial Reperfusion Injury (metabolism, physiopathology, prevention & control)
• Myocardium (metabolism)
• Oxidative Stress
• Rats
• Rats, Wistar
• Streptozocin (administration & dosage)
• Superoxide Dismutase (genetics, metabolism)
• Xanthine Oxidase (metabolism)