Abstract | OBJECTIVE: RESEARCH DESIGN AND METHODS: RESULTS: Cells lacking PAT proteins exhibited a dramatic increase in LD size and a decrease in LD number. Further, the lipolytic rate increased by approximately 2- to 2.5-fold in association with increased adipose triglyceride lipase (ATGL) at the LD surface. Downregulation of PAT proteins also produced insulin resistance, as indicated by decreased insulin stimulation of Akt phosphorylation (P < 0.001). Phosphoinositide-dependent kinase-1 and phosphoinositide 3-kinase decreased, and insulin receptor substrate-1 307 phosphorylation increased. Increased lipids in DIO mice livers were accompanied by changes in PAT composition but also increased ATGL, suggesting a relative PAT deficiency. CONCLUSIONS:
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Authors | Ming Bell, Hong Wang, Hui Chen, John C McLenithan, Da-Wei Gong, Rong-Zee Yang, Daozhan Yu, Susan K Fried, Michael J Quon, Constantine Londos, Carole Sztalryd |
Journal | Diabetes
(Diabetes)
Vol. 57
Issue 8
Pg. 2037-45
(Aug 2008)
ISSN: 1939-327X [Electronic] United States |
PMID | 18487449
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, N.I.H., Intramural, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Carrier Proteins
- Fatty Acids, Nonesterified
- Membrane Proteins
- Perilipin-2
- Perilipin-3
- Plin2 protein, mouse
- Plin3 protein, mouse
- RNA, Small Interfering
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Topics |
- Animals
- Carrier Proteins
(genetics, metabolism, physiology)
- Cells, Cultured
- Chromatography, Thin Layer
- Down-Regulation
- Fatty Acids, Nonesterified
(metabolism)
- Hepatocytes
(cytology, metabolism)
- Immunoblotting
- Immunohistochemistry
- Insulin Resistance
(physiology)
- Lipid Metabolism
(physiology)
- Lipolysis
(physiology)
- Membrane Proteins
(genetics, metabolism, physiology)
- Mice
- Mice, Inbred C57BL
- Microscopy, Confocal
- Perilipin-2
- Perilipin-3
- RNA, Small Interfering
(genetics)
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