Abstract | BACKGROUND: METHODS: Thirty sera from patients with WFA were tested: 14 with AEDS (group 1) and 16 with WDEIA, anaphylaxis or urticaria (group 2). An IgE Fc receptor (FcepsilonRI) humanized rat RBL-2H3 line was established by transfection with cDNAs encoding alpha-, beta- and gamma-subunits for the human IgE receptor. RESULTS: A humanized RBL clone was selected for its capacity to express mRNA alpha-, beta- and gamma-subunits of FcepsilonRI, to bind allergen-specific human IgE and to degranulate. In group 1, sera induced enhanced degranulation with AG extract, but rarely reacted with gliadins and glutenins. In group 2, half of the sera showed degranulation with LMW glutenins whereas the AG fraction and lipid transfer proteins were rarely positive. omega5-Gliadins did not appear as a major allergen in degranulation assays, although functional allergen-specific IgE was measurable in appreciable amounts. CONCLUSION: Our data demonstrate that in wheat food allergen evaluation, correlation exists between mast cell degranulation and IgE measurements, depending on the type of allergen. Therefore, the biological activity of some allergen types may also be affected by other parameters.
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Authors | M Bodinier, C Brossard, S Triballeau, M Morisset, C Guérin-Marchand, F Pineau, P de Coppet, D A Moneret-Vautrin, U Blank, S Denery-Papini |
Journal | International archives of allergy and immunology
(Int Arch Allergy Immunol)
Vol. 146
Issue 4
Pg. 307-20
( 2008)
ISSN: 1423-0097 [Electronic] Switzerland |
PMID | 18367844
(Publication Type: Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2008 S. Karger AG, Basel. |
Chemical References |
- MS4A2 protein, human
- RNA, Messenger
- Receptors, IgE
- Immunoglobulin E
- Gliadin
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Topics |
- Adult
- Animals
- Cell Degranulation
(immunology)
- Cell Line
- Dermatitis, Atopic
(immunology)
- Enzyme-Linked Immunosorbent Assay
- Flow Cytometry
- Fluorometry
- Gliadin
(immunology)
- Humans
- Immunoglobulin E
(analysis, immunology)
- Infant
- Mast Cells
(immunology)
- Middle Aged
- RNA, Messenger
(chemistry, genetics)
- Rats
- Receptors, IgE
(biosynthesis, genetics, immunology)
- Reverse Transcriptase Polymerase Chain Reaction
- Transfection
- Urticaria
(immunology)
- Wheat Hypersensitivity
(immunology)
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