Abstract | OBJECTIVE: Some authors suggest that growth factors are intermediate regulatory elements through which the ovarian hormones exert their growth-stimulatory effects on uterine leiomyomas. STUDY DESIGN: RESULTS: The Tris-HCl was more efficient at extracting solvent than 1M of acetic acid. Both myometrium and leiomyomas contained nanogram amounts of extractable TGF-beta and picogram amounts of PDGF. Western immunoblotting demonstrated that both factors exist as stable complexes, probably with extracellular matrix components. The PDGF/ TGF-beta ratio in Tris-HCl extracts was higher in leiomyomas than in myometrium and it increased during tumour growth. CONCLUSION: It is known that low concentrations of TGF-beta induce proliferation of cells by stimulating autocrine PDGF secretion. Higher concentrations of TGF-beta1 evoke a reverse effect by the down-regulation of the PDGF receptor and by direct growth inhibition. The increase in the PDGF/ TGF-beta ratio during tumour growth seems be important in tumour biology. The low amount of TGF-beta eliminates the inhibitory effect of this factor on cell proliferation and stimulates both autocrine PDGF secretion and promotes the synthesis of PDGF receptors. It is thus possible to bind more PDGF by myometrial cells resulting in a hyperplasia of myometrium and enhancement of extracellular matrix synthesis.
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Authors | Małgorzata Wolańska, Edward Bańkowski |
Journal | European journal of obstetrics, gynecology, and reproductive biology
(Eur J Obstet Gynecol Reprod Biol)
Vol. 130
Issue 2
Pg. 238-44
(Feb 2007)
ISSN: 0301-2115 [Print] Ireland |
PMID | 16564125
(Publication Type: Journal Article)
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Chemical References |
- Multiprotein Complexes
- Platelet-Derived Growth Factor
- Transforming Growth Factor beta
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Topics |
- Adult
- Extracellular Matrix
(metabolism, physiology)
- Female
- Humans
- Leiomyoma
(metabolism, pathology)
- Middle Aged
- Multiprotein Complexes
(metabolism)
- Myometrium
(metabolism)
- Platelet-Derived Growth Factor
(metabolism)
- Transforming Growth Factor beta
(metabolism)
- Uterine Neoplasms
(metabolism, pathology)
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