Enhanced soluble interleukin-5 receptor alpha expression in nasal polyposis.

Abstract

BACKGROUND:

Alternative splicing of the interleukin-5 receptor alpha (IL-5Ralpha)-subunit leads to the generation of a signalling, membrane-anchored (TM) isoform, or a secreted [soluble (SOL)], antagonistic variant. Given the key role of IL-5 in eosinophil function, we investigated SOL IL-5Ralpha expression pattern in an eosinophil-associated disease such as nasal polyposis (NP).

METHODS:

An SOL IL-5Ralpha enzyme-linked immunosorbent assay and quantitative real-time polymerase chain reaction (PCR) were established and applied in serum, nasal secretion and nasal tissue of controls (n = 12), and NP patients (n = 42) with or without asthma.

RESULTS:

Analysis of serum, nasal secretion, and nasal tissue samples revealed that SOL IL-5Ralpha protein concentrations were significantly increased in NP vs control tissue. Within the NP group, there was a significant up-regulation of SOL IL-5Ralpha in patients with systemic airway disease. These findings were confirmed at the mRNA level, using an optimized real-time reverse-transcriptase PCR procedure.

CONCLUSIONS:

This report demonstrates SOL IL-5Ralpha transcript and protein up-regulation in NP. Soluble IL-5Ralpha differentiates nasal polyps with or without concomitant asthma. As SOL IL-5Ralpha is strongly up-regulated for disease and has antagonistic properties in vitro, our studies shed new light on the mechanisms of specific immunomodulatory therapies, such as anti-IL-5.

• Authors: P Gevaert, C Bachert, G Holtappels, C P Novo, J Van der Heyden, L Fransen, S Depraetere, H Walter, P van Cauwenberge, J Tavernier
• Journal: Allergy (Allergy) Vol. 58 Issue 5 Pg. 371-9 (May 2003) ISSN: 0105-4538 [Print] Denmark
• PMID: 12752323 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Interleukin-5
• RNA, Messenger
• Receptors, Interleukin
• Receptors, Interleukin-5

Topics

• Adult
• Aged
• Antibodies, Monoclonal
• Asthma (immunology)
• Case-Control Studies
• Enzyme-Linked Immunosorbent Assay
• Eosinophils (immunology)
• Female
• Humans
• Interleukin-5 (blood, metabolism)
• Male
• Middle Aged
• Nasal Mucosa (immunology)
• Nasal Polyps (immunology)
• RNA, Messenger (blood)
• Receptors, Interleukin (blood, metabolism)
• Receptors, Interleukin-5
• Reverse Transcriptase Polymerase Chain Reaction
• Up-Regulation