Mapping the triphosphatase active site of baculovirus mRNA capping enzyme LEF4 and evidence for a two-metal mechanism.

Abstract

The 464-amino acid baculovirus LEF4 protein is a bifunctional mRNA capping enzyme with triphosphatase and guanylyltransferase activities. The N-terminal half of LEF4 constitutes an autonomous triphosphatase catalytic domain. The LEF4 triphosphatase belongs to a family of metal-dependent phosphohydrolases, which includes the RNA triphosphatases of fungi, protozoa, Chlorella virus and poxviruses. The family is defined by two glutamate-containing motifs (A and C), which form a metal-binding site. Most of the family members resemble the fungal and Chlorella virus enzymes, which have a complex active site located within the hydrophilic interior of a topologically closed eight stranded beta barrel (the so-called 'triphosphate tunnel'). Here we probed whether baculovirus LEF4 is a member of the tunnel subfamily, via mutational mapping of amino acids required for triphosphatase activity. We identified four new essential side chains in LEF4 via alanine scanning and illuminated structure-activity relationships by conservative substitutions. Our results, together with previous mutational data, highlight five acidic and four basic amino acids that are likely to comprise the LEF4 triphosphatase active site (Glu9, Glu11, Arg51, Arg53, Glu97, Lys126, Arg179, Glu181 and Glu183). These nine essential residues are conserved in LEF4 orthologs from all strains of baculoviruses. We discerned no pattern of clustering of the catalytic residues of the baculovirus triphosphatase that would suggest structural similarity to the tunnel proteins (exclusive of motifs A and C). However, there is similarity to the active site of vaccinia RNA triphosphatase. We infer that the baculovirus and poxvirus triphosphatases are a distinct lineage within the metal-dependent RNA triphosphatase family. Synergistic activation of the LEF4 triphosphatase by manganese and magnesium suggests a two-metal mechanism of gamma phosphate hydrolysis.

• Authors: Alexandra Martins, Stewart Shuman
• Journal: Nucleic acids research (Nucleic Acids Res) Vol. 31 Issue 5 Pg. 1455-63 (Mar 01 2003) ISSN: 1362-4962 [Electronic] England
• PMID: 12595553 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Viral Proteins
• late expression factor 4, baculovirus
• Manganese
• Nucleotidyltransferases
• mRNA guanylyltransferase
• Acid Anhydride Hydrolases
• RNA triphosphatase
• Magnesium

Topics

• Acid Anhydride Hydrolases (genetics, metabolism)
• Amino Acid Sequence
• Amino Acid Substitution
• Baculoviridae (enzymology)
• Binding Sites (genetics)
• Enzyme Activation (drug effects)
• Kinetics
• Magnesium (pharmacology)
• Manganese (pharmacology)
• Molecular Sequence Data
• Mutation
• Nucleotidyltransferases (genetics, metabolism)
• Sequence Homology, Amino Acid
• Viral Proteins (genetics, metabolism)