Decreased activity of anti-inflammatory
cytokines like
transforming growth factor (
TGF)-beta may contribute to allergic
inflammation. In vivo effects of
TGF-beta-effects are difficult to infer from local concentrations, since
TGF-beta-effects depend on a complex system of regulatory
proteins and receptors. Instead the effects of
TGF-beta might be inferred by examining
TGF-beta-inducible transcripts. In this study
DNA microarrays were used to examine local expression of
TGF-beta,
TGF-beta-regulatory and -inducible transcripts in nasal biopsies from patients with symptomatic
allergic rhinitis and healthy controls. In addition, nasal fluids were analysed with cytological and immunological methods. Nasal fluid eosinophils,
albumin, eosinophil granulae
proteins and
IgE, but not
TGF-beta, were higher in patients than in controls.
DNA microarray analysis of nasal mucosa showed expression of transcripts encoding
TGF-beta,
TGF-beta-regulatory
proteins and -receptors at variable levels in patients and controls. By comparison, analysis of 28
TGF-beta-inducible transcripts indicated that 23 of these had lower measurement values in patients than in controls, while one was higher, and the remaining four were absent in both patients and controls. In summary,
TGF-beta and a complex system of regulatory genes and receptors are expressed in the nasal mucosa. Low expression of
TGF-beta-inducible transcripts may indicate decreased
TGF-beta activity in
allergic rhinitis.
DNA microarray analysis may be a way to study
cytokine effects in vivo.