Abstract | OBJECTIVE: METHODS: For in vitro studies, hecate or hecate-beta hCG was added to cultures of ovarian cancer cells in the presence or absence of estradiol or follicle stimulating hormone. The cytotoxicity of lytic peptides was measured by trypan blue exclusion and lactate dehydrogenase release. For in vivo studies, OVCAR-3 xenografts were established in female athymic nude mice which were then treated once per week for 3 weeks with hecate or hecate-beta hCG via the lateral tail vein. An immunohistochemical method was used to analyze the expression of LH/hCG receptor in tumor and culture cells. RESULTS: In in vitro studies, both hecate-beta hCG and hecate destroyed ovarian cancer cells (NIH: OVCAR-3) in a dose-dependent manner. Removal of steroids from the culture medium reduced the sensitivity of the OVCAR-3 cell line to the hecate-beta hCG in a reversible manner. In in vivo studies, the average tumor volume and tumor burden in lytic peptide treated animals were reduced. In the groups of animals treated by hecate, hecate-beta hCG, and estradiol + hecate-beta hCG, tumor volumes after treatment expressed as a percentage of increase (197.4 +/- 21.72, 199.0 +/- 18.57, and 193.8 +/- 22.94%, respectively) were reduced, compared to control (263.0 +/- 21.72%) animals (P < 0.05). Immunocytochemical studies revealed the expression of LH/hCG receptor protein in the OVCAR-3 cells and tumor tissues. CONCLUSION:
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Authors | Barbara Gawronska, Carola Leuschner, Frederick M Enright, William Hansel |
Journal | Gynecologic oncology
(Gynecol Oncol)
Vol. 85
Issue 1
Pg. 45-52
(Apr 2002)
ISSN: 0090-8258 [Print] United States |
PMID | 11925119
(Publication Type: Journal Article)
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Chemical References |
- Chorionic Gonadotropin, beta Subunit, Human
- Receptors, LH
- hecate 1
- Melitten
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Topics |
- Animals
- Chorionic Gonadotropin, beta Subunit, Human
(administration & dosage, chemistry, pharmacology)
- Female
- Humans
- Immunohistochemistry
- Melitten
(administration & dosage, analogs & derivatives, chemistry, pharmacology)
- Mice
- Mice, Nude
- Ovarian Neoplasms
(drug therapy, metabolism)
- Receptors, LH
(biosynthesis)
- Tumor Cells, Cultured
- Xenograft Model Antitumor Assays
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