Effect of non-surgical periodontal therapy on C-telopeptide pyridinoline cross-links (ICTP) and interleukin-1 levels.

Abstract

BACKGROUND:

Biochemical markers harvested from gingival crevicular fluid (GCF) may be useful to identify and predict periodontal disease progression and to monitor the response to treatment. C-telopeptide pyridinoline cross-links (ICTP), a host-derived breakdown product specific for bone, and interleukin-1beta (IL-1), a potent bone-resorptive cytokine, have been associated with periodontal tissue destruction. The aim of this study was to examine the effect of non-surgical periodontal therapy on GCF levels of ICTP and IL-1.

METHODS:

Twenty-five chronic periodontitis subjects were monitored at 8 sites per subject at baseline prior to scaling and root planing and 1, 3, and 6 months after therapy. Four shallow (probing depths < 4 mm) and 4 deep (probing depths > or = 5 mm) sites were monitored for both marker levels and clinical parameters. GCF was collected for 30 seconds on paper strips, and levels of ICTP and IL-1 were determined using radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) techniques, respectively. Clinical measurements included probing depth (PD), clinical attachment level (CAL), and bleeding on probing (BOP).

RESULTS:

Deep sites exhibited significantly (P<0.001) higher ICTP and IL-1 levels compared to shallow sites at all time intervals. ICTP demonstrated a stronger association to clinical parameters than IL-1 including a modest correlation (r = 0.40, P<0.001) between ICTP and attachment loss. Significant improvements in PD, CAL, and BOP were observed at 1, 3, and 6 months in all sites (P<0.01). However, non-surgical mechanical therapy did not significantly reduce ICTP and IL-1 levels over the 6-month period. Further examination of subjects based on smoking status revealed that ICTP levels were significantly reduced at 3 and 6 months and IL-1 levels reduced at 3 months among non-smokers only.

CONCLUSIONS:

A single episode of non-surgical mechanical therapy did not significantly reduce biochemical markers associated with bone resorption in patients exhibiting chronic periodontitis. Future longitudinal studies are warranted to specifically evaluate the relationship between C-telopeptide pyridinoline cross-links and periodontal disease progression.

• Authors: K F Al-Shammari, W V Giannobile, W A Aldredge, V J Iacono, R M Eber, H L Wang, R J Oringer
• Journal: Journal of periodontology (J Periodontol) Vol. 72 Issue 8 Pg. 1045-51 (Aug 2001) ISSN: 0022-3492 [Print] United States
• PMID: 11525436 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Collagen Type I
• Interleukin-1
• Peptides
• collagen type I trimeric cross-linked peptide
• Collagen

Topics

• Adult
• Aged
• Alveolar Bone Loss (metabolism)
• Biomarkers
• Chronic Disease
• Collagen (analysis, metabolism)
• Collagen Type I
• Dental Scaling
• Disease Progression
• Enzyme-Linked Immunosorbent Assay
• Female
• Gingival Crevicular Fluid (chemistry, metabolism)
• Humans
• Interleukin-1 (analysis, metabolism)
• Male
• Middle Aged
• Peptides (analysis, metabolism)
• Periodontal Index
• Periodontitis (metabolism, therapy)
• Predictive Value of Tests
• Radioimmunoassay
• Smoking