Abstract | OBJECTIVE: MATERIALS AND METHODS: RESULTS: Leukemic cells from hairy cell leukemia variant and its cell line, with deletion of the Cepsilon gene, did not undergo IgE isotype switching in response to any of the stimuli. In contrast, a cell line (FH-5) established from chronic lymphocytic leukemia cells, bearing the Cepsilon gene, underwent the highest level of IgE isotype switching on stimulation with anti-CD40 mAb and IL-4. This response was correlated with the production of Cepsilon transcripts. IL-4, IL-10, and TNF-alpha induced higher levels of IgE isotype switching than the others. No IgE isotype switching was observed in any of the non-Hodgkin's lymphomas examined, except mantle cell lymphoma and lymphoplasmacytic lymphoma. Percentages of CD40(+) cells in five cases with follicular lymphoma were significantly lower than the other leukemias. CONCLUSIONS:
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Authors | H Takeuchi, H Kayano, T Hirose |
Journal | Experimental hematology
(Exp Hematol)
Vol. 28
Issue 5
Pg. 543-50
(May 2000)
ISSN: 0301-472X [Print] Netherlands |
PMID | 10812244
(Publication Type: Journal Article)
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Chemical References |
- Antibodies, Monoclonal
- CD40 Antigens
- Cytokines
- Immunoglobulin G
- Immunoglobulin E
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Topics |
- Antibodies, Monoclonal
(pharmacology)
- B-Lymphocytes
(drug effects, immunology)
- CD40 Antigens
(immunology)
- Cytokines
(pharmacology)
- Gene Deletion
- Genes, Immunoglobulin
- Humans
- Immunoglobulin Class Switching
- Immunoglobulin E
(genetics)
- Immunoglobulin G
(genetics)
- Immunophenotyping
- Leukemia, Hairy Cell
(genetics, immunology)
- Leukemia, Lymphocytic, Chronic, B-Cell
(classification, genetics, immunology)
- Reverse Transcriptase Polymerase Chain Reaction
- Transcription, Genetic
- Tumor Cells, Cultured
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