Abstract | BACKGROUND: A disruption of sebocyte differentiation and lipogenesis has fatal consequences and can cause a wide spectrum of skin diseases, from acne vulgaris to sebaceous carcinoma, however, the relevant molecular mechanisms have not been fully clarified. OBJECTIVES: The induction of autophagy and apoptosis in human sebocytes in response to biologically relevant fatty acids was investigated. METHODS: RESULTS:
Fatty acids induced the development of intracellular lipid droplets with subsequent apoptosis, whereas arachidonic acid caused the most rapid effect. Cleavage products of caspase-3 were only detected in arachidonic acid-induced apoptosis. The high basal apoptotic rate of cultured SZ95 sebocytes was strongly suppressed by QVD-Oph. Fatty acid-induced apoptosis was also markedly inhibited by QVD-Oph, whereas intracellular lipid droplets further accumulated. While cell viability after incubation with linoleic acid, palmitic acid, or palmitoleic acid and QVD-Oph was comparable with that of non-treated controls, arachidonic acid significantly reduced cell viability and cell density despite the concomitant pan- caspase inhibitor treatment. Using electron microscopy, typical autophagy structures were detected, such as autophagosomes and autolysosomes, at the basal level, which became more pronounced after treatment with fatty acids. CONCLUSIONS: Our findings contribute to a better understanding of the inflammation-associated mechanisms of lipogenesis and cell death induction in human sebocytes and may help to unveil the effects of fatty acid-rich human nutrition.
|
Authors | Amir M Hossini, Xiaoxiao Hou, Tarik Exner, Beatrix Fauler, Jürgen Eberle, Anja Rabien, Evgenia Makrantonaki, Christos C Zouboulis |
Journal | Skin pharmacology and physiology
(Skin Pharmacol Physiol)
Vol. 36
Issue 1
Pg. 1-15
( 2023)
ISSN: 1660-5535 [Electronic] Switzerland |
PMID | 36384913
(Publication Type: Journal Article)
|
Copyright | © 2022 S. Karger AG, Basel. |
Chemical References |
- Fatty Acids, Nonesterified
- Palmitic Acid
- Caspases
- Arachidonic Acids
|
Topics |
- Humans
- Fatty Acids, Nonesterified
(pharmacology, metabolism)
- Sebaceous Glands
- Palmitic Acid
(pharmacology, metabolism)
- Apoptosis
- Caspases
(metabolism, pharmacology)
- Autophagy
- Arachidonic Acids
(metabolism, pharmacology)
|