Abstract | BACKGROUND AND PURPOSE: EXPERIMENTAL APPROACH: CNV was induced in mice with laser photocoagulation. Choroid/retinal pigment epithelium (RPE) complexes and MΦ were isolated for analysis by qRT-PCR, western blot, flow cytometry, immunostaining, metabolic measurements and angiogenesis assays. KEY RESULTS: MΦ accumulated within the CNV of murine nAMD models and expressed high levels of glycolysis-related enzymes and M1/M2 polarization markers. This phenotype of hyper-glycolytic and activated MΦ was replicated in bone marrow-derived macrophages stimulated by necrotic RPE in vitro. Myeloid cell-specific knockout of PFKFB3, a key glycolytic activator, attenuated pathological neovascularization in laser-induced CNV, which was associated with decreased expression of MΦ polarization markers and pro-angiogenic factors, along with decreased sprouting of vessels in choroid/RPE complexes. Mechanistically, necrotic RPE increased PFKFB3-driven glycolysis in macrophages, leading to activation of HIF-1α/HIF-2α and NF-κB, and subsequent induction of M1/M2 markers and pro-angiogenic cytokines, finally promoting macrophage reprogramming towards an angiogenic phenotype to facilitate development of CNV. The PFKFB3 inhibitor AZ67 also inhibited activation of HIF-1α/HIF-2α and NF-κB signalling and almost completely prevented laser-induced CNV in mice. CONCLUSIONS AND IMPLICATIONS: Modulation of PFKFB3-mediated macrophage glycolysis and activation is a promising strategy for the treatment of nAMD.
|
Authors | Zhiping Liu, Xiaoxiao Mao, Qiuhua Yang, Xiaoyu Zhang, Jiean Xu, Qian Ma, Yaqi Zhou, Qingen Da, Yongfeng Cai, Anu Sopeyin, Zheng Dong, Mei Hong, Ruth B Caldwell, Akrit Sodhi, Yuqing Huo |
Journal | British journal of pharmacology
(Br J Pharmacol)
Vol. 179
Issue 22
Pg. 5109-5131
(11 2022)
ISSN: 1476-5381 [Electronic] England |
PMID | 35830274
(Publication Type: Journal Article)
|
Copyright | © 2022 The Authors. British Journal of Pharmacology published by John Wiley & Sons Ltd on behalf of British Pharmacological Society. |
Chemical References |
- Basic Helix-Loop-Helix Transcription Factors
- Cytokines
- NF-kappa B
- PFKFB3 protein, mouse
- Phosphofructokinase-2
- Phosphoric Monoester Hydrolases
- Glucose
|
Topics |
- Animals
- Basic Helix-Loop-Helix Transcription Factors
(metabolism)
- Choroidal Neovascularization
(etiology, metabolism, prevention & control)
- Cytokines
(metabolism)
- Disease Models, Animal
- Glucose
- Glycolysis
- Mice
- Mice, Inbred C57BL
- NF-kappa B
(metabolism)
- Phosphofructokinase-2
- Phosphoric Monoester Hydrolases
|