Paper-Based Flow Sensor for the Detection of Hyaluronidase via an Enzyme Hydrolysis-Induced Viscosity Change in a Polymer Solution.

Abstract

Hyaluronidase (HAase) is implicated in inflammation, cancer development, and allergic reaction. The detection of HAase is significantly important in clinical diagnosis and medical treatment. Herein, we propose a new principle for the development of equipment-free and label-free paper-based flow sensors based on the enzymatic hydrolysis-induced viscosity change in a stimuli-responsive polymer solution, which increases the water flow distance on the pH indicator paper. The detection of HAase is demonstrated as an example. This facile and versatile method can overcome the potential drawbacks of traditional hydrogel-based sensors, including complex preparation steps, slow response time, or low sensitivity. Moreover, it can also avoid the use of specialized instruments, labeled molecules, or functionalized nanoparticles in the sensors developed using the polymer solutions. Using this strategy, the detection of HAase is achieved with a limit of detection as low as 0.2 U/mL. Also, it works well in human urine. Additionally, the detection of tannic acid, which is an inhibitor of HAase, is also fulfilled. Overall, a simple, efficient, high-throughput, and low-cost detection method is developed for the rapid and quantitative detection of HAase and its inhibitor without the use of labeled molecules, synthetic particles, and specialized instruments. As only minimal reagents of HAase, HA, and paper are used, it is very promising in the development of commercial kits for point-of-care testing.

• Authors: Binglu Zhao, Lubin Qi, Wenjun Tai, Mei Zhao, Xiangfeng Chen, Li Yu, Jianguo Shi, Xiao Wang, Jin-Ming Lin, Qiongzheng Hu
• Journal: Analytical chemistry (Anal Chem) Vol. 94 Issue 11 Pg. 4643-4649 (03 22 2022) ISSN: 1520-6882 [Electronic] United States
• PMID: 35258931 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Polymers
• Hyaluronic Acid
• Hyaluronoglucosaminidase

Topics

• Humans
• Hyaluronic Acid (chemistry)
• Hyaluronoglucosaminidase (urine)
• Hydrolysis
• Polymers
• Viscosity