Abstract | BACKGROUND:
Diabetic retinopathy (DR) is a serious complication of diabetes. Numerous reports have validated that circular RNAs ( circRNAs) participate in DR progression. This study aimed to elucidate the role and potential mechanism of circRNA zinc finger protein 532 (circZNF532) in DR. METHODS: The levels of circZNF532, miR-1243, and coactivator associated arginine methyltransferase 1 ( CARM1) in DR patients and human retinal microvascular endothelial cells (hRMECs) were determined by quantitative real-time PCR and western blot. Colony formation assay, transwell assay, tube formation assay and enzyme-linked immunosorbent assay were used to assess the biological function of hRMECs. The binding relationship between miR-1243 and circZNF532/ CARM1 was verified by dual- luciferase reporter and RNA immunoprecipitation assays. RESULTS: circZNF532 and CARM1 levels were increased, while miR-1243 level was reduced in DR patients and high glucose (HG)-stimulated hRMECs. In terms of mechanism, miR-1243 competitively bound to circZNF532 and CARM1. Down-regulation of circZNF532 restrained HG-induced hRMECs proliferation, migration, invasion, angiogenesis and inflammation via regulating miR-1243. In addition, miR-1243 inhibited HG-triggered hRMECs progression via targeting CARM1. CONCLUSION: circZNF532 facilitated HG-induced angiogenesis and inflammation in hRMECs via modulating the miR-1243/ CARM1 pathway, suggesting that circZNF532 might be a potential biomarker for DR treatment.
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Authors | Ting Wang, Chaopeng Li, Min Shi, Shi Zhou, Jiajing Chen, Fang Wang |
Journal | Diabetology & metabolic syndrome
(Diabetol Metab Syndr)
Vol. 14
Issue 1
Pg. 14
(Jan 21 2022)
ISSN: 1758-5996 [Print] England |
PMID | 35063035
(Publication Type: Journal Article)
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Copyright | © 2022. The Author(s). |