Sericin protects against acute sleep deprivation-induced memory impairment via enhancement of hippocampal synaptic protein levels and inhibition of oxidative stress and neuroinflammation in mice.

Abstract

Sleep deprivation (SD) induces learning and memory deficits via inflammatory responses and oxidative stress. On the other hand, sericin (Ser) possesses potent antioxidant and neuroprotective effects. We investigated the effect of different doses of Ser on the SD-induced cognitive impairment. Ser (100, 200, and 300 mg/kg) was administered to animals via oral gavage for 8 days, 5 days before to SD, and during SD. SD was induced in mice using a modified multiple platform model, starting on the 6th day for 72 h. Spatial learning and memory were assessed using the Lashley III maze. Serum corticosterone level, and hippocampal malondialdehyde (MDA), total antioxidant capacity (TAC), and the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPx) enzymes were evaluated. The expression of growth-associated protein 43 (GAP-43), post-synaptic density-95 (PSD-95), synapsin 1 (SYN-1), and synaptophysin (SYP), and inflammation markers were detected by western blotting. SD caused cognitive impairment, while Ser pretreatment prevented such an effect. Serum corticosterone also increased with SD, but its levels were suppressed in SD mice receiving Ser. Furthermore, Ser normalized SD-induced reduction in the hippocampus activity of SOD and GPx, increased TAC, and decreased MDA levels. Besides, Ser pretreatment increased GAP-34, SYP, SYN-I, and PSD-95 and reduced IL1-β and TNF-α in the hippocampus. SD induced memory impairment and pretreatment with Ser improved memory via its antioxidant, anti-inflammation, and up-regulation of synaptic proteins in the hippocampus.

• Authors: Fereshteh Farajdokht, Seyed Mehdi Vatandoust, Leila Hosseini, Kiarash Fekri, Sepideh Rahigh Aghsan, Alireza Majdi, Saeed Sadigh-Eteghad, Javad Mahmoudi
• Journal: Brain research bulletin (Brain Res Bull) Vol. 174 Pg. 203-211 (09 2021) ISSN: 1873-2747 [Electronic] United States
• PMID: 34153383 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2021 Elsevier Inc. All rights reserved.

Chemical References

• Antioxidants
• Cytokines
• Sericins
• Corticosterone

Topics

• Animals
• Antioxidants (metabolism, pharmacology)
• Cognition Disorders (drug therapy, etiology, psychology)
• Corticosterone (blood)
• Cytokines (metabolism)
• Hippocampus (metabolism)
• Male
• Maze Learning (drug effects)
• Memory Disorders (drug therapy, etiology, psychology)
• Mice
• Mice, Inbred C57BL
• Neuroinflammatory Diseases (drug therapy, etiology)
• Oxidative Stress (drug effects)
• Sericins (therapeutic use)
• Sleep Deprivation (complications, psychology)
• Synapses (metabolism)
• Up-Regulation (drug effects)