Fluvastatin, a traditional fat-decreasing
drug, is widely used for curing
cardiovascular disease. Previous reports demonstrated that
fluvastatin pretreatment protected against
myocardial ischemia/reperfusion (I/R) by inhibiting TLR4 signaling pathway and/or reducing proinflammatory
cytokines. However, whether
fluvastatin has a cardioprotective effect against apoptosis and autophagy remains unknown. This study aims to evaluate whether the cardioprotective role of
fluvastatin in I/R is mediated by high-mobility group box 1 (
HMGB1)/
toll-like receptor 4 (TLR4) pathway via anti-apoptotic and anti-autophagic functions. Sprague-Dawley rats were anesthetized, artificially ventilated and subjected to 30 min of
coronary occlusion, followed by 4 h of reperfusion. The animals were randomized into four groups: (i)
Sham operation; (ii) I/R; (iii) I/R + low-dosage
fluvastatin (10 mg/kg); and (iv) I/R + high-dosage
fluvastatin (20 mg/kg). After reperfusion, the hemodynamic parameters,
myocardial infarct size, structural alteration of myocardium, apoptosis index, pro-inflammatory
cytokine production,
Beclin-1, Light chain 3 (LC3),
HMGB1, TLR4 and
Nuclear factor kappa B (NF-κB)
protein levels were measured and recorded. It was found that
fluvastatin preconditioning improved
left ventricular dysfunction, reduced
HMGB1/TLR4/NF-κB expressions, and inhibited cardiomyocyte apoptosis, autophagy, and
inflammation reaction. Moreover, treatment with
fluvastatin ameliorated myocardial injury by reducing
infarct size, causing less damage to cardiac structure, downregulating
autophagy-related protein expression and releasing pro-
inflammation mediators. Our findings indicate that
fluvastatin exerts beneficial effects on cardiac ischemic damage, which may be associated with its anti-autophagic and anti-apoptotic functions via inhibition of
HMGB1/TLR4-related pathway during I/R injury.