Supplementation with ribonucleotide-based ingredient (Ribodiet®) lessens oxidative stress, brain inflammation, and amyloid pathology in a murine model of Alzheimer.

Abstract

Alzheimer's disease (AD) is the most common type of dementia worldwide, characterized by the deposition of neurofibrillary tangles and amyloid-β (Aβ) peptides in the brain. Additionally, increasing evidence demonstrates that a neuroinflammatory state and oxidative stress, iron-dependent, play a crucial role in the onset and disease progression. Besides conventional therapies, the use of natural-based products represents a future medical option for AD treatment and/or prevention. We, therefore, evaluated the effects of a ribonucleotides-based ingredient (Ribodiet®) in a non-genetic mouse model of AD. To this aim, mice were injected intracerebroventricularly (i.c.v.) with Aβ1-42 peptide (3 µg/3 μl) and after with Ribodiet® (0.1-10 mg/mouse) orally (p.o.) 3 times weekly for 21 days following the induction of experimental AD. The mnemonic and cognitive decline was then evaluated, and, successively, we have assessed ex vivo the modulation of different cyto-chemokines on mice brain homogenates. Finally, the level of GFAP, S100β, and iron-related metabolic proteins were monitored as markers of reactive gliosis, neuro-inflammation, and oxidative stress. Results indicate that Ribodiet® lessens oxidative stress, brain inflammation, and amyloid pathology via modulation of iron-related metabolic proteins paving the way for its rationale use for the treatment of AD and other age-related diseases.

• Authors: Anella Saviano, Gian Marco Casillo, Federica Raucci, Alessia Pernice, Cristina Santarcangelo, Marialuisa Piccolo, Maria Grazia Ferraro, Miriam Ciccone, Alessandro Sgherbini, Nadia Pedretti, Daniele Bonvicini, Carlo Irace, Maria Daglia, Nicola Mascolo, Francesco Maione
• Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (Biomed Pharmacother) Vol. 139 Pg. 111579 (Jul 2021) ISSN: 1950-6007 [Electronic] France
• PMID: 33845375 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 The Authors. Published by Elsevier Masson SAS.. All rights reserved.

Chemical References

• Amyloid beta-Peptides
• Biomarkers
• Nonheme Iron Proteins
• Peptide Fragments
• Ribonucleotides
• amyloid beta-protein (1-42)

Topics

• Alzheimer Disease (prevention & control, psychology)
• Amyloid beta-Peptides
• Animals
• Behavior, Animal (drug effects)
• Biomarkers
• Cerebral Amyloid Angiopathy (prevention & control, psychology)
• Diet
• Dietary Supplements
• Encephalitis (prevention & control, psychology)
• Gliosis (prevention & control)
• Injections, Intraventricular
• Male
• Mice
• Nonheme Iron Proteins (metabolism)
• Oxidative Stress (drug effects)
• Peptide Fragments
• Psychomotor Performance (drug effects)
• Ribonucleotides (pharmacology, therapeutic use)