Exosomal miRNAs Profile in Children's Nonalcoholic Fatty Liver Disease and the Correlation with Transaminase and Uric Acid.

Abstract

OBJECTIVE:

The incidence of non-alcoholic fatty liver disease (NAFLD) in children increased rapidly. However, the pathogenesis of NAFLD, especially how non-alcoholic fatty liver progress to non-alcoholic steatohepatitis, is still unclear. This study aims to explore the exosomal miRNAs profiles and the underline pathogenesis of child NAFLD.

METHODS:

Twenty NAFLD and 20 health control were enrolled in this study. Circulating exosomes were isolated, and RNA sequencing was performed in test set (3 NAFLD/3 Controls). The differentially expressed miRNAs (DEM) were further validated in validation set (17 NAFLD/17 Controls). Spearman correlation -analysis was used to investigate the association between DEM and clinical parameters.

RESULTS:

Eighty-two miRNAs were differentially expressed (absolute fold change >2 and p < 0.05) in the 2 groups, they were involved in fat acid metabolism, starch and sucrose metabolism, bile acid metabolism and inflammation. miRNA122-5p, miRNA34a-5p, -miRNA155-5p and miRNA146b-3p were up-regulated in NAFLD group (p < 0.05) and positively correlated with body mass index (r, 0.41-0.59), alanine aminotransferase (r, 0.36-0.52), aspartate transaminase (r, 0.31-0.48) and uric acid (UA, r, 0.51-0.69; p < 0.05).

CONCLUSIONS:

Circulating exosomal miRNAs may be involved in the pathogenesis of NAFLD and correlated with transaminase and UA.

• Authors: Xuelian Zhou, Ke Huang, Junjun Jia, Yan Ni, Jinna Yuan, Xinyi Liang, Hu Lin, Wei Peng, Wei Wu, Guanping Dong, Junfen Fu
• Journal: Annals of nutrition & metabolism (Ann Nutr Metab) Vol. 76 Issue 1 Pg. 44-53 ( 2020) ISSN: 1421-9697 [Electronic] Switzerland
• PMID: 32172249 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2020 S. Karger AG, Basel.

Chemical References

• MIRN122 microRNA, human
• MIRN155 microRNA, human
• MicroRNAs
• Uric Acid
• Aspartate Aminotransferases
• Alanine Transaminase

Topics

• Alanine Transaminase
• Aspartate Aminotransferases
• Child
• Humans
• Liver (metabolism)
• MicroRNAs (genetics)
• Non-alcoholic Fatty Liver Disease (pathology)
• Uric Acid