Theacrine alleviates chronic inflammation by enhancing TGF-β-mediated shifts via TGF-β/SMAD pathway in Freund's incomplete adjuvant-induced rats.

Abstract

Rheumatoid arthritis is a chronic and systemic autoimmune disease, which affects approximately 1% of the adult population worldwide. The present study investigated the therapeutic effect of theacrine (TC) on arthritis and its mechanisms in Freund's incomplete adjuvant (FIA)-induced SD rats. Rats were randomly divided into 5 groups: i) healthy control; ii) model; iii) positive control with methotrexate (MTX); iv) treatment with 12.5 mg/kg TC; and v) treatment with 25.0 mg/kg TC. The apparent scores, including changes in body weights, degree of paw swelling and arthritis indicators, were analyzed to evaluate the anti-chronic inflammatory effect of TC. The levels of interleukin (IL)-6 and transforming growth factor-β (TGF-β) in serum were measured by enzyme-linked immunosorbent assay. The protein and RNA expression levels of the critical factors in rats were measured to elucidate the mechanisms responsible for chronic inflammation and to verify molecular indexes of chronic inflammatory conditions. TC notably suppressed the severity of FIA-induced rat by attenuating the apparent scores, animal weight and inflammatory indexes in the 25 mg/kg TC group compared with the FIA rat model. Furthermore, TC significantly decreased the levels of IL-6 and increased the levels of TGF-β. Histopathological examinations indicated that TC rescued the synovial hyperplasia and inflammatory cell infiltration in joint tissues. In addition, TC enhanced TGF-β-mediated shifts in inflammatory marker expression in joint tissue. Overall, the present study demonstrated that TC exerted a superior anti-arthritic effect via the suppression of IL-6 and the activation of TGF-β by the TGF-β/SMAD pathway.

• Authors: Ming Gao, Jun Zheng, Chao Zheng, Zhiyuan Huang, Qinwei Huang
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 522 Issue 3 Pg. 743-748 (02 12 2020) ISSN: 1090-2104 [Electronic] United States
• PMID: 31791581 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Lipids
• Smad Proteins
• Transforming Growth Factor beta
• incomplete Freund's adjuvant
• Uric Acid
• Freund's Adjuvant
• 1,3,7,9-tetramethyluric acid

Topics

• Animals
• Anti-Inflammatory Agents (therapeutic use)
• Arthritis, Experimental (chemically induced, drug therapy, immunology, pathology)
• Arthritis, Rheumatoid (chemically induced, drug therapy, immunology, pathology)
• Chronic Disease
• Freund's Adjuvant
• Inflammation (chemically induced, drug therapy, immunology, pathology)
• Joints (drug effects, immunology, pathology)
• Lipids
• Rats
• Rats, Sprague-Dawley
• Signal Transduction (drug effects)
• Smad Proteins (analysis, immunology)
• Transforming Growth Factor beta (analysis, immunology)
• Uric Acid (analogs & derivatives, therapeutic use)