Perilla oil regulates intestinal microbiota and alleviates insulin resistance through the PI3K/AKT signaling pathway in type-2 diabetic KKAy mice.

Abstract

Perilla oil (PerO), a natural oil with a high unsaturated fatty acid content derived from the mature seeds of Perilla frutescens, is a homology of medicine and food. The type 2 diabetes mellitus (T2DM) model was successfully established using a high-fat and high-sugar diet combined with a single low-dose of streptozocin (STZ). PerO intervention reduced the levels of fasting blood glucose and the level, size and accumulation of lipid droplets, increased the insulin level and diminished the body weight loss. PerO pretreatment markedly promoted the serum levels of alanine transaminase (ALT) and aspartate transaminase alanine (AST) and inhibited the levels of glucose (GLU), glucose-6-phosphate dehydrogenase (G6PD), triglycerides (TGs) and total cholesterol (TC). Moreover, PerO treatment enhanced the expression of phosphoinositide-3 kinase (PI3K)/protein kinase B (AKT) and activated the expression of glucose transporter 4 (Glut4) and phospho-AKT serine/threonine kinase (p-AS160) in the liver. Additionally, PerO treatment distinctly decreased the abundance of Aerococcus and facilitated the richness of Alloprevotella in the intestine, as well as accelerated the restoration of the gut microflora diversity. Thus, PerO regulates intestinal microbiota and alleviates insulin resistance through the PI3K/AKT signaling pathway in type-2 diabetic KKAy mice and may be a potential functional food for diabetic treatment.

• Authors: Jiankang Wang, Yutong He, Deqing Yu, Liang Jin, Xiaobao Gong, Baoshun Zhang
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 135 Pg. 110965 (Jan 2020) ISSN: 1873-6351 [Electronic] England
• PMID: 31743741 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 Elsevier Ltd. All rights reserved.

Chemical References

• Blood Glucose
• Hypoglycemic Agents
• Insulin
• Plant Oils
• alpha-Linolenic Acid
• Streptozocin
• perilla seed oil
• Phosphatidylinositol 3-Kinase
• Proto-Oncogene Proteins c-akt

Topics

• Animals
• Blood Glucose (metabolism)
• Body Weight (drug effects)
• Diabetes Mellitus, Experimental (chemically induced, drug therapy, physiopathology)
• Diabetes Mellitus, Type 2 (chemically induced, drug therapy, physiopathology)
• Gastrointestinal Microbiome (drug effects)
• Hypoglycemic Agents (therapeutic use)
• Insulin (metabolism)
• Insulin Resistance (physiology)
• Liver (drug effects, pathology)
• Male
• Mice, Inbred C57BL
• Muscle, Skeletal (drug effects, pathology)
• Perilla (chemistry)
• Phosphatidylinositol 3-Kinase (metabolism)
• Plant Oils (therapeutic use)
• Proto-Oncogene Proteins c-akt (metabolism)
• Signal Transduction (drug effects)
• Streptozocin
• alpha-Linolenic Acid (therapeutic use)