Selenoprotein W deficiency does not affect oxidative stress and insulin sensitivity in the skeletal muscle of high-fat diet-fed obese mice.

Abstract

Selenoprotein W (SelW) is a selenium-containing protein with a redox motif found abundantly in the skeletal muscle of rodents. Previous in vitro studies suggest that SelW plays an antioxidant role; however, relatively few in vivo studies have addressed the antioxidant role of SelW. Since oxidative stress is a causative factor for the development of insulin resistance in obese subjects, we hypothesized that if SelW plays a role as an antioxidant, SelW deficiency could aggravate the oxidative stress and insulin resistance caused by a high-fat diet. SelW deficiency did not affect insulin sensitivity and H2O2 levels in the skeletal muscle of control diet-fed mice. SelW levels in the skeletal muscle were decreased by high-fat diet feeding for 12 wk. High-fat diet induced obesity and insulin resistance and increased the levels of H2O2 and oxidative stress makers, which were not affected by SelW deficiency. High-fat diet feeding increased the expression of antioxidant enzymes; however, SelW deficiency did not affect the expression levels of antioxidants. These results suggest that SelW does not play a protective role against oxidative stress and insulin resistance in the skeletal muscle of high-fat diet-fed obese mice.

• Authors: Min-Gyeong Shin, Hye-Na Cha, Soyoung Park, Yong-Woon Kim, Jong-Yeon Kim, So-Young Park
• Journal: American journal of physiology. Cell physiology (Am J Physiol Cell Physiol) Vol. 317 Issue 6 Pg. C1172-C1182 (12 01 2019) ISSN: 1522-1563 [Electronic] United States
• PMID: 31509445 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Selenoprotein W
• Hydrogen Peroxide
• Catalase
• Sod1 protein, mouse
• Superoxide Dismutase
• Superoxide Dismutase-1
• superoxide dismutase 2

Topics

• Animals
• Catalase (genetics, metabolism)
• Diet, High-Fat (adverse effects)
• Gene Expression Regulation
• Glucose Tolerance Test
• Humans
• Hydrogen Peroxide (metabolism)
• Insulin Resistance
• Male
• Mice
• Mice, Inbred C57BL
• Mice, Knockout
• Muscle, Skeletal (metabolism, pathology)
• Obesity (etiology, genetics, metabolism, pathology)
• Oxidative Stress
• Selenoprotein W (deficiency, genetics)
• Superoxide Dismutase (genetics, metabolism)
• Superoxide Dismutase-1 (genetics, metabolism)