Vitamin D Deficiency in Rats Causes Cardiac Dysfunction by Inducing Myocardial Insulin Resistance.

Abstract

SCOPE:

Cause-effect relationship between vitamin D deficiency and cardiometabolic abnormalities remains undefined. The aim is to investigate the role of vitamin D deficiency in cardiac failure, through possible involvement in myocardial insulin signaling.

METHODS AND RESULTS:

Male SD rats (n = 6) are fed a normal diet (Con), vitamin D-deficient diet [Con(-)], or high-fat, high fructose diet (HFHFrD) for 20 weeks. Cardiac hypertrophy and fetal gene program are confirmed in Con(-) group. Cardiac dysfunction is assessed by echocardiography. Elevated renin, TGF-β and collagen-1α mRNAs, p-ERK1/2, and perivascular fibrosis indicate cardiac remodeling in Con(-) group. Increased serum insulin, triglycerides, and blood pressure, and decreased glucose tolerance and HDL cholesterol are observed in Con(-) rats. Decreased p-Akt/Akt, GLUT4, SOD2, and catalase, and increased NF-κB, TNF-α, and IL-6 are observed in Con(-) hearts. In H9c2 cells, calcitriol attenuates palmitate-induced insulin resistance. VDR-silenced H9c2 cells show reduced Akt phosphorylation, GLUT4 translocation, and 2-NBDG uptake. Findings in Con(-) and HFHFrD groups are comparable.

CONCLUSION:

Vitamin D deficiency in rats mimic high-fat-, high-fructose-induced metabolic syndrome and cardiac dysfunction. This study demonstrates that vitamin D deficiency is an independent risk factor for heart failure, at least in part, through induction of myocardial insulin resistance.

• Authors: Hina Lateef Nizami, Parmeshwar Katare, Pankaj Prabhakar, Yashwant Kumar, Sudheer Kumar Arava, Praloy Chakraborty, Subir Kumar Maulik, Sanjay Kumar Banerjee
• Journal: Molecular nutrition & food research (Mol Nutr Food Res) Vol. 63 Issue 17 Pg. e1900109 (09 2019) ISSN: 1613-4133 [Electronic] Germany
• PMID: 31095894 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Receptors, Calcitriol
• Renin
• Glucose

Topics

• Animals
• Cardiomegaly
• Dyslipidemias (etiology)
• Gene Expression Regulation
• Glucose (metabolism)
• Heart (physiopathology)
• Hyperinsulinism (etiology)
• Hypertension (etiology)
• Hypertrophy, Left Ventricular (etiology)
• Insulin Resistance
• Male
• Myocardium (metabolism)
• Rats, Sprague-Dawley
• Receptors, Calcitriol (genetics)
• Renin (genetics)
• Ventricular Remodeling
• Vitamin D Deficiency (complications, genetics, physiopathology)