Abstract | OBJECTIVE: The aim of this study was to clarify the expression of Ninj1 in endometriosis and adenomyosis lesions, and its inductive factor in human endometriotic stromal cells (ESCs). BACKGROUND: Nerve injury-induced protein 1 (Ninj1) is a molecule originally identified in dorsal root ganglion neurons and Schwann cells after nerve injury and promotes neurite outgrowth. The aim of this study was to clarify the expression of Ninj1 in endometriosis and adenomyosis lesions, and its inductive factor in human endometriotic stromal cells (ESCs). MATERIALS AND METHODS: Tissues were obtained with consent from patients diagnosed with ovarian endometrioma (n = 15 in total), peritoneal endometriosis (n = 5), adenomyosis (n = 5), and other gynecological disorders (n = 5, control) during surgery. Immunohistochemistry was conducted in order to detect Ninj1 protein expression in the lesion of endometriosis, adenomyosis, and eutopic endometrium. Nerve fibers in the ovarian endometrioma were detected by positive staining of PGP-9.5. To evaluate the effects of IL-1β on Ninj1 gene expression in endometriosis, ESCs isolated from ovarian endometrioma (n = 5) were treated with IL-1β (5 ng/mL) for 3 or 6 hours. Messenger RNA ( mRNA) expression for Ninj1 was examined using quantitative RT-PCR. RESULTS: CONCLUSION:
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Authors | Mariko Miyashita, Kaori Koga, Arisa Takeuchi, Tomoko Makabe, Ayumi Taguchi, Yoko Urata, Gentaro Izumi, Masashi Takamura, Miyuki Harada, Tetsuya Hirata, Yasushi Hirota, Osamu Wada-Hiraike, Osamu Yoshino, Tomoyuki Fujii, Yutaka Osuga |
Journal | Reproductive sciences (Thousand Oaks, Calif.)
(Reprod Sci)
Vol. 26
Issue 8
Pg. 1105-1110
(08 2019)
ISSN: 1933-7205 [Electronic] United States |
PMID | 30326781
(Publication Type: Journal Article)
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Chemical References |
- Cell Adhesion Molecules, Neuronal
- Interleukin-1beta
- NINJ1 protein, human
- Nerve Growth Factors
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Topics |
- Adenomyosis
(metabolism)
- Cell Adhesion Molecules, Neuronal
(metabolism)
- Endometriosis
(metabolism)
- Endometrium
(drug effects, metabolism)
- Female
- Gene Expression Regulation
(drug effects)
- Humans
- Immunohistochemistry
- Inflammation
(metabolism)
- Interleukin-1beta
(pharmacology)
- Nerve Growth Factors
(metabolism)
- Ovarian Diseases
(metabolism)
- Peritoneal Diseases
(metabolism)
- Stromal Cells
(drug effects, metabolism)
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