Protective roles of estradiol against vascular oxidative stress in ovariectomized female rats exposed to normoxia or intermittent hypoxia.

Abstract

AIM:

We tested the hypothesis that estradiol (E2 ) reduces aortic oxidative stress and endothelial dysfunction in ovariectomized (OVX) female rats exposed to room air (RA) or chronic intermittent hypoxia (CIH).

METHODS:

We used intact or OVX female rats treated with vehicle or E2 (0.5 mg/kg/d) and exposed to RA or CIH (21%-10% O2 , 10 cycles/h, 8 h/d) for 7 or 35 days, and measured the arterial pressure, heart rate and plasma endothelin-1 levels. We also measured in thoracic aortic samples, the activities of the pro-oxidant enzymes NADPH (NOX) and xanthine oxidase (XO), the antioxidant enzymes superoxide dismutase, catalase, glutathione peroxidase and the advanced oxidation protein products (AOPP-oxidative stress marker). Finally, we used aortic rings to assess the contractile response to phenylephrine and the vasodilatory response to acetylcholine.

RESULTS:

After 7 or 35 days of CIH, E2 supplementation reduced arterial pressure. E2 reduced plasma endothelin-1 levels after 7 days of CIH, but not after 35 days. Ovariectomy, but not CIH for 7 days, increased aortic oxidative stress and E2 treatment prevented this effect. Remarkably, in animals exposed to RA, this was achieved by a reduction in NOX and XO activities, but in animals exposed to CIH this was achieved by increased catalase activity. In OVX female rats exposed to CIH for 7 days, E2 supplementation improved the NO-mediated vasodilation. After 35 days of CIH, enzymatic activities, AOPP and aortic reactivity were similar in all groups.

CONCLUSION:

E2 -based therapy could help prevent the vascular consequences of CIH in apneic women.

• Authors: Alexandra Ribon-Demars, Vincent Pialoux, Anaëlle Boreau, François Marcouiller, Richard Larivière, Aida Bairam, Vincent Joseph
• Journal: Acta physiologica (Oxford, England) (Acta Physiol (Oxf)) Vol. 225 Issue 2 Pg. e13159 (02 2019) ISSN: 1748-1716 [Electronic] England
• PMID: 29947475 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2018 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd.

Chemical References

• Endothelin-1
• Estrogens
• Reactive Oxygen Species
• Estradiol
• Oxygen

Topics

• Animals
• Aorta (enzymology)
• Arterial Pressure (physiology)
• Endothelin-1 (blood)
• Endothelium, Vascular (drug effects, physiopathology)
• Estradiol (pharmacology)
• Estrogens (pharmacology)
• Female
• Hypoxia (blood, enzymology, physiopathology)
• Ovariectomy
• Oxidative Stress (drug effects)
• Oxygen (pharmacology)
• Rats
• Rats, Sprague-Dawley
• Reactive Oxygen Species (metabolism)
• Vasodilation (drug effects)