Chlorogenic acid suppresses lipopolysaccharide‑induced nitric oxide and interleukin‑1β expression by inhibiting JAK2/STAT3 activation in RAW264.7 cells.

Abstract

Chlorogenic acid (CA) is a phenolic compound purified from coffee, fruits and their associated beverages, which possess various biological properties, such as antioxidant and anticarcinogenic activities. The present study evaluated the effects of CA on lipopolysaccharide (LPS)‑induced inflammation in RAW264.7 cells and the associated intracellular signaling pathways using reverse transcription‑quantitative polymerase chain reaction, western blotting and enzyme‑linked immunosorbent assays. CA pretreatment inhibited LPS‑induced expression of inducible nitric oxide synthase (iNOS), nitric oxide (NO) and pro‑inflammatory mediators including interleukin (IL)‑6, tumor necrosis factor‑α (TNF‑α), macrophage inflammatory protein‑2 (MIP‑2) and IL‑1β in RAW264.7 cells. In addition, phosphorylation of Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) with LPS was inhibited by CA pretreatment. CA and STAT3 inhibitor (STAT3i) pretreatment inhibited LPS‑induced nuclear translocation of phosphorylated STAT3. In addition, STAT3i inhibited the LPS‑induced expression of iNOS, NO and IL‑1β similar to the results of CA pretreatment. By contrast, STAT3i did not inhibit the LPS‑induced increase in IL‑6, TNF‑α and MIP‑2 expression. These results indicate that CA may suppress LPS‑induced NO and IL‑1β expression by inhibiting JAK2/STAT3 activation in RAW264.7 cells.

• Authors: Sang-Hun Kim, Sun-Young Park, Young-Lan Park, Dae-Seong Myung, Jong-Sun Rew, Young-Eun Joo
• Journal: Molecular medicine reports (Mol Med Rep) Vol. 16 Issue 6 Pg. 9224-9232 (Dec 2017) ISSN: 1791-3004 [Electronic] Greece
• PMID: 28990048 (Publication Type: Journal Article)

Chemical References

• Cytokines
• Inflammation Mediators
• Interleukin-1beta
• Lipopolysaccharides
• STAT3 Transcription Factor
• Chlorogenic Acid
• Nitric Oxide
• Janus Kinase 2

Topics

• Animals
• Chlorogenic Acid (pharmacology)
• Cytokines (metabolism)
• Gene Expression Regulation (drug effects)
• Inflammation Mediators (metabolism)
• Interleukin-1beta (genetics, metabolism)
• Janus Kinase 2 (metabolism)
• Lipopolysaccharides (immunology)
• Macrophages (drug effects, immunology, metabolism)
• Mice
• Models, Biological
• Nitric Oxide (metabolism)
• Protein Binding
• Protein Transport
• RAW 264.7 Cells
• STAT3 Transcription Factor (metabolism)
• Signal Transduction (drug effects)