The aim of the current study was to elucidate the effect of seabuckthorn leaves (SL) extract and
flavonoid glycosides extract from seabuckthorn leaves (SLG) on diet-induced
obesity and related metabolic disturbances, and additionally, to identify whether
flavonoid glycosides and other components in SL can exert a possible interaction for the prevention of
metabolic diseases by comparing the effect of SL and SLG. C57BL/6J mice were fed a normal diet (ND, AIN-93G purified diet), high-fat diet (HFD, 60 kcal% fat), HFD + 1.8% (w/w) SL (SL), and HFD + 0.04% (w/w) SLG (SLG) for 12 weeks. In high fat-fed mice, SL and SLG decreased the adiposity by suppressing lipogenesis in adipose tissue, while increasing the energy expenditure. SL and SLG also improved hepatic steatosis by suppressing hepatic lipogenesis and
lipid absorption, whilst also enhancing hepatic
fatty acid oxidation, which may be linked to the improvement in
dyslipidemia. Moreover, SL and SLG improved
insulin sensitivity by suppressing the levels of plasma GIP that were modulated by secreted
resistin and pro-inflammatory
cytokine, and hepatic glucogenic
enzyme activities. SL, especially its
flavonoid glycosides (SLG), can protect against the deleterious effects of diet-induced
obesity (DIO) and its metabolic complications such as adiposity,
dyslipidemia,
inflammation, hepatic steatosis, and
insulin resistance.