Effect of breviscapine against hepatic ischemia reperfusion injury.

Abstract

BACKGROUND:

Breviscapine is an active ingredient extracted from traditional Chinese medicine Erigeron breviscapus. The purpose of this study was to investigate the effect of breviscapine injection on hepatic ischemia and/or reperfusion injury.

METHODS:

Forty rats were randomly divided into five groups (n = 8): Sham group, Ischemia reperfusion 1 (I/R1) + normal saline (NS) group, I/R1 + breviscapine (Bre), I/R2 + NS group, and I/R2 + Bre group. Group1 and group2 represent ischemia time for 10 min and 30 min, respectively. Breviscapine or normal saline was administered to rats (single dose of 10 mg/Kg, intravenously) 30 min before hepatic ischemia. Serum transaminases, histopathologic changes, malondialdehyde (MDA), and superoxide dismutase (SOD) in liver tissues were evaluated. The expression level of mitochondrial fusion 2 (Mfn2) was also investigated.

RESULTS:

After 24-h reperfusion, based on the histopathologic analysis, compared with NS control group, the liver function was improved in breviscapine group. Liver enzymes aspartate and alanine aminotransferase levels were significantly lower in the I/R + Bre group, when compared with the I/R + NS group. Pretreatment with breviscapine reduced MDA level (P < 0.05) and increased SOD activity significantly in I/R + Bre compared with I/R + NS group. Western blot and RT-q polymerase chain reaction showed that Mfn2 was significantly downregulated in breviscapine preconditioning group as compared to normal saline control group.

CONCLUSIONS:

Breviscapine preconditioning attenuates liver ischemia reperfusion injury via inhibiting liver oxidative stress reaction. The protective mechanism probably inhibits Mfn2 protein and mRNA expression.

• Authors: Yan-Zhu Lin, Zhi-Yuan Lu, Xiao-Hui Liang, Kang Li, Bo Peng, Jin Gong
• Journal: The Journal of surgical research (J Surg Res) Vol. 203 Issue 2 Pg. 268-74 (06 15 2016) ISSN: 1095-8673 [Electronic] United States
• PMID: 27363631 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 Elsevier Inc. All rights reserved.

Chemical References

• Biomarkers
• Drugs, Chinese Herbal
• Flavonoids
• Protective Agents
• breviscapine

Topics

• Animals
• Biomarkers (metabolism)
• Blotting, Western
• Drug Administration Schedule
• Drugs, Chinese Herbal (pharmacology, therapeutic use)
• Flavonoids (pharmacology, therapeutic use)
• Injections, Intravenous
• Liver (drug effects, metabolism, pathology)
• Male
• Oxidative Stress (drug effects)
• Protective Agents (pharmacology, therapeutic use)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Real-Time Polymerase Chain Reaction
• Reperfusion Injury (metabolism, pathology, prevention & control)
• Treatment Outcome