Abstract |
Vanins are enzymes that convert pantetheine to pantothenic acid ( vitamin B5). Insights into the function of vanins have evolved lately, indicating vanin-1 to play a role in inflammation, oxidative stress and cell migration. Moreover, vanin-1 has recently gained attention as a novel modulator of hepatic glucose and lipid metabolism. In the present study, we investigated the role of vanin-1 in the development of hepatic steatosis and insulin resistance in animal models of obesity and diabetes. In addition, we evaluated the potency of RR6, a novel pharmacological vanin-1 inhibitor, as an anti-diabetic drug. Increased vanin activity was observed in plasma and liver of high fat diet (HFD)-induced obese mice, as well as ZDF-diabetic rats. Ablation of vanin-1 (Vnn1(-/-) mice) mildly improved glucose tolerance and insulin sensitivity in HFD-fed mice, but had no effects on body weight, hepatic steatosis or circulating lipid levels. Oral administration of RR6 for 8 days completely inhibited plasma vanin activity, but did not affect hepatic glucose production, insulin sensitivity or hepatic steatosis in ZDF-diabetes rats. In conclusion, absence of vanin-1 activity improves insulin sensitivity in HFD-fed animals, yet short-term inhibition of vanin activity may have limited value as an anti-diabetic strategy.
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Authors | Janna A van Diepen, Patrick A Jansen, Dov B Ballak, Anneke Hijmans, Floris P J T Rutjes, Cees J Tack, Mihai G Netea, Joost Schalkwijk, Rinke Stienstra |
Journal | Scientific reports
(Sci Rep)
Vol. 6
Pg. 21906
(Mar 02 2016)
ISSN: 2045-2322 [Electronic] England |
PMID | 26932716
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Enzyme Inhibitors
- GPI-Linked Proteins
- Insulin
- Lipids
- Amidohydrolases
- Vnn1 protein, rat
- pantetheinase
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Topics |
- Amidohydrolases
(antagonists & inhibitors, genetics, metabolism)
- Animals
- Diabetes Mellitus, Experimental
(blood, enzymology, pathology)
- Diabetes Mellitus, Type 2
(blood, enzymology, pathology)
- Diet, High-Fat
- Disease Models, Animal
- Enzyme Inhibitors
(pharmacology)
- GPI-Linked Proteins
(antagonists & inhibitors, genetics, metabolism)
- Insulin
(pharmacology)
- Insulin Resistance
- Lipids
(blood)
- Male
- Mice
- Mice, Knockout
- Obesity
(blood, enzymology, pathology)
- Rats, Wistar
- Rats, Zucker
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