Abstract | BACKGROUND: METHOD:
Colon cancer chemopreventive potential of hydro- methanol extract of Rumex abyssinicus rhizome was determined based on the number and multiplicity of aberrant crypt foci (ACF). Fifteen DMH (1, 2-dimethylhydrazine) treated and five untreated Wistar female rats were used. DMH was administered subcutaneously 30 mg/kg, after its pH was adjusted to 6.5-7. Treatment groups started receiving extract after six weeks of weekly DMH injections. The rats were divided in to four groups: Group 1 received only oral normal saline, Group 2 received DMH and normal saline, Group 3 and 4 received DMH plus 250 mg/kg and 500 mg/kg extract, respectively. Specific phytoconstituents of the plant, which were reviewed from original articles, were virtually evaluated for cyclooxygenase-2 (COX-2) inhibition. The binding energies and interactions of the phytochemicals from Rumex abyssinicus against COX-2 were determined by Autodock4.2. RESULTS: There was a statistically significant reduction (p-value < 0.05) in the number of aberrant crypt (AC) and aberrant crypt foci (ACF) at both administered doses. However, significant association (p-value > 0.05) was not observed in reducing crypt multiplicity. The docking process resulted in estimated binding energies [-6.83 kcal/mol to -7.9 kcal/mol] which are closer to the positive controls or Non-Steroidal Anti-Inflammatory Drugs ( NSAIDs) [-4.55 kcal/mol to -10.84 kcal/mol]. The phytochemical-COX-2 interaction indicated the involvement of key amino acid residues in inhibition of cyclooxygenase like ARG120, TYR355, TYR385, SER530 and GLY526. CONCLUSIONS: Rumex abyssinicus had demonstrated a chemopreventive potential at post-initiation stage. As the virtual screening data suggested, COX-2 inhibition by the anthraquinones in the extract could be one mechanism for the observed chemopreventive effect.
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Authors | Biniyam Girma, Getnet Yimer, Eyasu Makonnen |
Journal | BMC complementary and alternative medicine
(BMC Complement Altern Med)
Vol. 15
Pg. 365
(Oct 15 2015)
ISSN: 1472-6882 [Electronic] England |
PMID | 26470793
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents, Phytogenic
- Cyclooxygenase 2 Inhibitors
- Plant Extracts
- 1,2-Dimethylhydrazine
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Topics |
- 1,2-Dimethylhydrazine
(toxicity)
- Animals
- Antineoplastic Agents, Phytogenic
(pharmacology, therapeutic use)
- Chemoprevention
- Colonic Neoplasms
(genetics, pathology, prevention & control)
- Cyclooxygenase 2 Inhibitors
(pharmacology, therapeutic use)
- DNA Damage
- Female
- Phytotherapy
- Plant Extracts
(pharmacology, therapeutic use)
- Rats
- Rats, Wistar
- Rumex
(chemistry)
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