Liposomes Combined an Integrin αvβ3-Specific Vector with pH-Responsible Cell-Penetrating Property for Highly Effective Antiglioma Therapy through the Blood-Brain Barrier.

Abstract

Glioma, one of the most common aggressive malignancies, has the highest mortality in the present world. Delivery of nanocarriers from the systemic circulation to the glioma sites would encounter multiple physiological and biological barriers, such as blood-brain barrier (BBB) and the poor penetration of nanocarriers into the tumor. To circumvent these hurdles, the paclitaxel-loaded liposomes were developed by conjugating with a TR peptide (PTX-TR-Lip), integrin αvβ3-specific vector with pH-responsible cell-penetrating property, for transporting drug across the BBB and then delivering into glioma. Surface plasmon resonance (SPR) studies confirmed the very high affinity of TR-Lip and integrin αvβ3. In vitro results showed that TR-Lip exhibited strong transport ability across BBB, killed glioma cells and brain cancer stem cells (CSCs), and destroyed the vasculogenic mimicry (VM) channels. In vivo results demonstrated that TR-Lip could better target glioma, and eliminated brain CSCs and the VM channels in tumor tissues. The median survival time of tumor-bearing mice after administering PTX-TR-Lip (45 days) was significantly longer than that after giving free PTX (25.5 days, p < 0.001) or other controls. In conclusion, PTX-TR-Lip would improve the therapeutic efficacy of brain glioma in vitro and in vivo.

• Authors: Kairong Shi, Yang Long, Chaoqun Xu, Yang Wang, Yue Qiu, Qianwen Yu, Yayuan Liu, Qianyu Zhang, Huile Gao, Zhirong Zhang, Qin He
• Journal: ACS applied materials & interfaces (ACS Appl Mater Interfaces) Vol. 7 Issue 38 Pg. 21442-54 (Sep 30 2015) ISSN: 1944-8252 [Electronic] United States
• PMID: 26371468 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Integrin alphaVbeta3
• Liposomes
• Peptides
• Paclitaxel

Topics

• Animals
• Antineoplastic Agents (pharmacology, therapeutic use)
• Biological Transport (drug effects)
• Blood-Brain Barrier (drug effects, metabolism)
• Brain Neoplasms (pathology)
• Cell Death (drug effects)
• Cell Line
• Cell Survival (drug effects)
• Diagnostic Imaging
• Electric Impedance
• Endocytosis (drug effects)
• Epithelium (drug effects)
• Female
• Glioma (drug therapy, pathology)
• Hydrogen-Ion Concentration
• Integrin alphaVbeta3 (metabolism)
• Liposomes
• Mice, Inbred BALB C
• Neoplastic Stem Cells (drug effects)
• Paclitaxel (pharmacology, therapeutic use)
• Particle Size
• Peptides (chemistry)
• Proton Magnetic Resonance Spectroscopy
• Spheroids, Cellular (drug effects, pathology)
• Static Electricity
• Surface Plasmon Resonance