Preventive effects of oleuropein against cardiac remodeling after myocardial infarction in Wistar rat through inhibiting angiotensin-converting enzyme activity.

Abstract

OBJECTIVE:

Myocardial infarction remains the major cause of global death due to cardiovascular diseases. This study aimed to assess the protective role of oleuropein in attenuating the cardiac remodeling in isoproterenol-induced myocardial infarction in rats.

METHODS AND RESULTS:

Male Wistar rats were randomly divided into four groups, control, isoproterenol (Isop) and pretreated animals with oleuropein at two different doses (20 and 40 mg/kg) orally for 7 days and intoxicated with isoproterenol (Isop+Oleu20) and (Isop+Oleu40) groups. The subcutaneous injection of isoproterenol (100 mg/kg body weight) to untreated rats for two consecutive days showed significant increases in ST-segment elevation, heart weight index and alteration in the ECG pattern and hemodynamic function. Else, serum levels of cardiac troponin-T, creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH) and alanine aminotransferase (ALT) underwent a notable rise in serum of Isop group by (345, 82, 73 and 106%, respectively) as compared to normal rats. Isoproterenol-induced myocardial injury was evidenced by alteration in serum lipids profile and increased activities of pancreatic lipase by 94% and angiotensin-converting enzyme (ACE) by 78% which reflects the occurrence of cardiac remodeling process. The histopathological findings of the infarcted group showed myocardium necrosis and cells inflammatory infiltration. However, the treatment with oleuropein gave a good protection of the myocardium by decreasing cardiac injury markers specially troponin-T, restoring hemodynamic parameters and attenuating cardiac remodeling process through inhibition of ACE activity.

CONCLUSION:

Oleuropein offers high preventive effects from cardiac remodeling process in rats with acute myocardial infarction.

• Authors: Kais Mnafgui, Ines Khlif, Raouf Hajji, Fatma Derbali, Ftouma Kraiem, Hedi Ellefi, Thomas Michel, Maria Halabalaki, Alexios Leandros Skaltsounis, Abdelfattah Elfeki, Neji Gharsallah, Noureddine Allouche
• Journal: Toxicology mechanisms and methods (Toxicol Mech Methods) Vol. 25 Issue 7 Pg. 538-46 ( 2015) ISSN: 1537-6524 [Electronic] England
• PMID: 26056852 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adrenergic beta-Agonists
• Angiotensin-Converting Enzyme Inhibitors
• Iridoid Glucosides
• Iridoids
• Vasodilator Agents
• oleuropein
• Isoproterenol

Topics

• Adrenergic beta-Agonists
• Angiotensin-Converting Enzyme Inhibitors (therapeutic use)
• Animals
• Dose-Response Relationship, Drug
• Electrocardiography (drug effects)
• Heart Function Tests
• Hemodynamics (drug effects)
• Iridoid Glucosides
• Iridoids (isolation & purification, therapeutic use)
• Isoproterenol
• Male
• Myocardial Infarction (chemically induced, pathology, prevention & control)
• Olea (chemistry)
• Plant Roots (chemistry)
• Rats
• Rats, Wistar
• Vasodilator Agents (therapeutic use)
• Ventricular Remodeling (drug effects)