Class I and II histone deacetylase expression in human chronic periodontitis gingival tissue.

Abstract

BACKGROUND AND OBJECTIVE:

Histone deacetylase inhibitors (HDACi) are being considered to treat chronic inflammatory diseases at low doses. Currently HDACi that are more specific are being developed to target particular HDACs; therefore, this study aimed to determine levels and distribution of class I and II HDAC in human gingival samples obtained from patients with chronic periodontitis.

MATERIAL AND METHODS:

Gingival biopsies were obtained from patients with and without (mild inflammation, no bone loss) periodontitis. Total RNA was isolated for real-time quantitative polymerase chain reaction to determine expression of HDACs 1-10. Immunohistochemistry was used to determine protein distribution of HDACs 1, 5, 8 and 9. Factor VIII, CD3 and tartrate resistant acid phosphatase (TRAP) were detected in serial sections to identify blood vessels, lymphocytes, pre-osteoclasts and osteoclasts cells respectively. Tumour necrosis factor α (TNF-α) expression was also assessed.

RESULTS:

mRNA for HDAC 1, 5, 8 and 9 were significantly upregulated in chronic periodontitis gingival tissues compared to non-periodontitis samples (p < 0.05). Significantly higher HDAC 1 protein expression was observed in chronic periodontitis samples (p < 0.05), and was associated with CD3, TRAP and TNF-α-positive cells. HDAC 1, 5, 8 and 9 were expressed strongly by the factor VIII-positive microvasculature in the chronic periodontitis gingival tissues.

CONCLUSIONS:

HDAC 1, 5, 8 and 9 expression was higher in gingival tissues from patients with chronic periodontitis compared to non-periodontitis samples. Results suggest that these HDACs could therefore be targeted with specific acting HDACi.

• Authors: M D Cantley, A A S S K Dharmapatni, K Algate, T N Crotti, P M Bartold, D R Haynes
• Journal: Journal of periodontal research (J Periodontal Res) Vol. 51 Issue 2 Pg. 143-51 (Apr 2016) ISSN: 1600-0765 [Electronic] United States
• PMID: 26031835 (Publication Type: Journal Article)
• Copyright: © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Chemical References

• Tumor Necrosis Factor-alpha
• Histone Deacetylases

Topics

• Chronic Periodontitis
• Gingiva
• Histone Deacetylases
• Humans
• Osteoclasts
• Tumor Necrosis Factor-alpha