Abstract | AIMS: METHODS AND RESULTS: Mitochondrial damage, cardiomyocyte apoptosis, and cardiac dysfunction after doxorubicin administration were significantly attenuated in mice with cardiac-specific overexpression of HMGB1 (HMGB1-Tg) compared with wild type (WT) -mice. HSPB1 levels after doxorubicin administration were significantly higher in HMGB1-Tg mice than in WT mice. Transfection with HMGB1 increased the expression of HSPB1 at both the protein and mRNA levels, and HMGB1 inhibited mitochondrial dysfunction and apoptosis after exposure of cardiomyocytes to doxorubicin. HSPB1 silencing abrogated the inhibitory effect of HMGB1 on cardiomyocyte apoptosis. Doxorubicin increased the binding of HMGB1 to heat shock factor 2 and enhanced heat shock element promoter activity. Moreover, HMGB1 overexpression greatly enhanced heat shock element promoter activity. Silencing of heat shock factor 2 attenuated HMGB1-dependent HSPB1 expression and abrogated the ability of HMGB1 to suppress cleaved caspase-3 accumulation after doxorubicin stimulation. CONCLUSIONS: We report the first in vivo and in vitro evidence that cardiac HMGB1 increases HSPB1 expression and attenuates cardiomyocyte apoptosis associated with doxorubicin-induced cardiomyopathy. Cardiac HMGB1 increases HSPB1 expression in cardiomyocytes in a heat shock factor 2-dependent manner.
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Authors | Taro Narumi, Tetsuro Shishido, Yoichiro Otaki, Shinpei Kadowaki, Yuki Honda, Akira Funayama, Shintaro Honda, Hiromasa Hasegawa, Daisuke Kinoshita, Miyuki Yokoyama, Satoshi Nishiyama, Hiroki Takahashi, Takanori Arimoto, Takuya Miyamoto, Tetsu Watanabe, Atsushi Tanaka, Chang-Hoon Woo, Jun-ichi Abe, Yasuchika Takeishi, Isao Kubota |
Journal | Journal of molecular and cellular cardiology
(J Mol Cell Cardiol)
Vol. 82
Pg. 1-12
(May 2015)
ISSN: 1095-8584 [Electronic] England |
PMID | 25736854
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015. Published by Elsevier Ltd. |
Chemical References |
- HMGB1 Protein
- HSP27 Heat-Shock Proteins
- Doxorubicin
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Topics |
- Animals
- Animals, Newborn
- Apoptosis
(genetics)
- Cardiomyopathies
(chemically induced, diagnosis, genetics, metabolism, mortality)
- Cell Line
- Cells, Cultured
- Doxorubicin
(administration & dosage, adverse effects, pharmacology)
- Gene Expression Regulation
- HMGB1 Protein
(genetics, metabolism)
- HSP27 Heat-Shock Proteins
(genetics)
- Humans
- Membrane Potential, Mitochondrial
- Mice
- Mice, Transgenic
- Mitochondria
(metabolism)
- Myocardium
(metabolism)
- Myocytes, Cardiac
(drug effects, metabolism, ultrastructure)
- Promoter Regions, Genetic
- Rats
- Transcriptional Activation
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