Abstract | BACKGROUND: OBJECTIVES: The aim of this study was to determine the combined effect of gallic acid and CsA on antioxidant capacity of isolated heart tissues during ischemia reperfusion. MATERIALS AND METHODS: EIGHTY MALE WISTAR RATS WERE RANDOMLY ASSIGNED TO DIFFERENT GROUPS: sham, control (Ca, received saline, 1 mL/kg); 3 groups were pretreated with gallic acid (G1a: 7.5, G2a: 15, G3a: 30 mg/kg) for 10 days, and the other 3 groups were pretreated with gallic acid and received CsA (0.2 µM) for 10 minutes before induction of ischemia and during the first 10 minutes of reperfusion (G1b, G2b and G3b) and the last group received CsA alone (Cb). After 10 days of pretreatment, the heart was isolated and transferred to the Langendorff apparatus and exposed to 30 minutes ischemia followed by 60 minutes of reperfusion. After that cardiac markers and antioxidant enzymes were assessed in cardiac tissues. RESULTS: CONCLUSIONS:
Gallic acid and CsA combination improved antioxidant capacity and cell membrane integrity more than each one alone. Therefore, it can be a therapeutic approach to reduce the I/R injury.
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Authors | Mohammad Badavi, Najmeh Sadeghi, Mahin Dianat, Alireza Samarbafzadeh |
Journal | Iranian Red Crescent medical journal
(Iran Red Crescent Med J)
Vol. 16
Issue 6
Pg. e16424
(Jun 2014)
ISSN: 2074-1804 [Print] Estonia |
PMID | 25068044
(Publication Type: Journal Article)
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