Abstract | BACKGROUND: METHODS: GAD65 messenger RNA ( mRNA) levels were quantified in PFC area 9 by quantitative polymerase chain reaction from 62 subjects with schizophrenia or schizoaffective disorder and 62 matched healthy comparison subjects. In a subset of subject pairs, GAD65 relative protein levels were quantified by confocal immunofluorescence microscopy. RESULTS: CONCLUSIONS: In concert with previous studies, these findings suggest that schizoaffective disorder is associated with lower levels of both GAD65 and GAD67 mRNA and protein in the PFC, whereas subjects with schizophrenia have lower mean levels of only GAD67 mRNA and protein. Because cognitive function is generally better preserved in patients with schizoaffective disorder relative to patients with schizophrenia, these findings may support an interpretation that GAD65 downregulation provides a homeostatic response complementary to GAD67 downregulation that serves to reduce inhibition in the face of lower PFC network activity.
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Authors | Jill R Glausier, Sohei Kimoto, Kenneth N Fish, David A Lewis |
Journal | Biological psychiatry
(Biol Psychiatry)
Vol. 77
Issue 2
Pg. 167-76
(Jan 15 2015)
ISSN: 1873-2402 [Electronic] United States |
PMID | 24993056
(Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Society of Biological Psychiatry. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- RNA, Messenger
- Glutamate Decarboxylase
- glutamate decarboxylase 2
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Topics |
- Cohort Studies
- Female
- Glutamate Decarboxylase
(genetics, metabolism)
- Humans
- Immunohistochemistry
- Male
- Microscopy, Confocal
- Microscopy, Fluorescence
- Middle Aged
- Polymerase Chain Reaction
- Prefrontal Cortex
(enzymology)
- Psychotic Disorders
(enzymology)
- RNA, Messenger
(genetics, metabolism)
- Schizophrenia
(enzymology)
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