Magnolia extract (BL153) protection of heart from lipid accumulation caused cardiac oxidative damage, inflammation, and cell death in high-fat diet fed mice.

Abstract

Magnolia as an herbal material obtained from Magnolia officinalis has been found to play an important role in anti-inflammation, antioxidative stress, and antiapoptosis. This study was designed to investigate the effect of Magnolia extract (BL153) on obesity-associated lipid accumulation, inflammation, oxidative stress, and apoptosis in the heart. C57BL/6 mice were fed a low- (10 kcal% fat) or high-fat (60 kcal% fat) diet for 24 weeks to induce obesity. These mice fed with high-fat diet (HFD) were given a gavage of vehicle, 2.5, 5, or 10 mg/kg body weight BL153 daily. The three doses of BL153 treatment slightly ameliorated insulin resistance without decrease of body weight gain induced by HFD feeding. BL153 at 10 mg/kg slightly attenuated a mild cardiac hypertrophy and dysfunction induced by HFD feeding. Both 5 mg/kg and 10 mg/kg of BL153 treatment significantly inhibited cardiac lipid accumulation measured by Oil Red O staining and improved cardiac inflammation and oxidative stress by downregulating ICAM-1, TNF-α, PAI-1, 3-NT, and 4-HNE. TUNEL staining showed that BL153 treatment also ameliorated apoptosis induced by mitochondrial caspase-3 independent cell death pathway. This study demonstrates that BL153 attenuates HFD-associated cardiac damage through prevention of HFD-induced cardiac lipid accumulation, inflammation, oxidative stress, and apoptosis.

• Authors: Weixia Sun, Zhiguo Zhang, Qiang Chen, Xia Yin, Yaowen Fu, Yang Zheng, Lu Cai, Ki-Soo Kim, Ki Ho Kim, Yi Tan, Young Heui Kim
• Journal: Oxidative medicine and cellular longevity (Oxid Med Cell Longev) Vol. 2014 Pg. 205849 ( 2014) ISSN: 1942-0994 [Electronic] United States
• PMID: 24693333 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Cardiotonic Agents
• Plant Extracts
• Triglycerides

Topics

• Animals
• Biomarkers (metabolism)
• Body Weight (drug effects)
• Cardiotonic Agents (pharmacology, therapeutic use)
• Cell Death (drug effects)
• Diet, High-Fat (adverse effects)
• Endoplasmic Reticulum Stress (drug effects)
• Energy Intake (drug effects)
• Feeding Behavior (drug effects)
• Heart Function Tests
• Inflammation (drug therapy, pathology)
• Insulin Resistance
• Lipid Metabolism (drug effects)
• Magnolia (chemistry)
• Male
• Mice
• Mice, Inbred C57BL
• Mitochondria (drug effects, metabolism)
• Myocardium (metabolism, pathology)
• Organ Size (drug effects)
• Oxidative Stress (drug effects)
• Phytotherapy
• Plant Extracts (pharmacology, therapeutic use)
• Signal Transduction (drug effects)
• Triglycerides (blood)