Nanoparticle formulation enhanced protective immunity provoked by PYGPI8p-transamidase related protein (PyTAM) DNA vaccine in Plasmodium yoelii malaria model.

Abstract

We have previously reported the new formulation of polyethylimine (PEI) with gamma polyglutamic acid (γ-PGA) nanoparticle (NP) to have provided Plasmodium yoelii merozoite surface protein-1 (PyMSP-1) plasmid DNA vaccine with enhanced protective cellular and humoral immunity in the lethal mouse malaria model. PyGPI8p-transamidase-related protein (PyTAM) was selected as a possible candidate vaccine antigen by using DNA vaccination screening from 29 GPI anchor and signal sequence motif positive genes picked up using web-based bioinformatics tools; though the observed protection was not complete. Here, we observed augmented protective effect of PyTAM DNA vaccine by using PEI and γ-PGA complex as delivery system. NP-coated PyTAM plasmid DNA immunized mice showed a significant survival rate from lethal P. yoelii challenge infection compared with naked PyTAM plasmid or with NP-coated empty plasmid DNA group. Antigen-specific IgG1 and IgG2b subclass antibody levels, proportion of CD4 and CD8T cells producing IFN-γ in the splenocytes and IL-4, IFN-γ, IL-12 and TNF-α levels in the sera and in the supernatants from ex vivo splenocytes culture were all enhanced by the NP-coated PyTAM DNA vaccine. These data indicates that NP augments PyTAM protective immune response, and this enhancement was associated with increased DC activation and concomitant IL-12 production.

• Authors: Mahamoud Sama Cherif, Mohammed Nasir Shuaibu, Yukinobu Kodama, Tomoaki Kurosaki, Gideon Kofi Helegbe, Mihoko Kikuchi, Akitoyo Ichinose, Tetsuo Yanagi, Hitoshi Sasaki, Katsuyuki Yui, Nguyen Huy Tien, Juntra Karbwang, Kenji Hirayama
• Journal: Vaccine (Vaccine) Vol. 32 Issue 17 Pg. 1998-2006 (Apr 07 2014) ISSN: 1873-2518 [Electronic] Netherlands
• PMID: 24440206 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Ltd. All rights reserved.

Chemical References

• Antibodies, Protozoan
• Malaria Vaccines
• Protozoan Proteins
• Recombinant Proteins
• Vaccines, DNA
• poly(gamma-glutamic acid)
• Interleukin-12
• Polyglutamic Acid
• Interferon-gamma
• Polyethyleneimine

Topics

• Animals
• Antibodies, Protozoan (blood)
• Cells, Cultured
• Dendritic Cells (immunology)
• Female
• Immunity, Cellular
• Immunity, Humoral
• Interferon-gamma (immunology)
• Interleukin-12 (immunology)
• Malaria (prevention & control)
• Malaria Vaccines (genetics, immunology)
• Mice
• Mice, Inbred C57BL
• Nanoparticles
• Plasmodium yoelii (immunology)
• Polyethyleneimine (chemistry)
• Polyglutamic Acid (analogs & derivatives, chemistry)
• Protozoan Proteins (immunology)
• Recombinant Proteins (immunology)
• Spleen (cytology, immunology)
• T-Lymphocytes (immunology)
• Vaccines, DNA (immunology)