Abstract | BACKGROUND:
Elastin is a signature protein of the arteries and lungs, thus it was hypothesized that elastin is subject to enzymatic degradation during cardiovascular and pulmonary diseases. The aim was to investigate if different fragments of the same protein entail different information associated to two different diseases and if these fragments have the potential of being diagnostic biomarkers. METHODS: RESULTS: ELM and ELM-2 neoepitopes were both localized in diseased carotid arteries and fibrotic lungs. In the cardiovascular cohort, ELM-2 levels were 66% higher in serum from AMI patients compared to patients with no AMI (p<0.01). Levels of ELM were not significantly increased in these patients and no correlation was observed between ELM-2 and ELM. ELM-2 was not elevated in the COPD and IPF patients and was not correlated to ELM. ELM was shown to be correlated with smoking habits (p<0.01). CONCLUSIONS: The ELM-2 neoepitope was related to AMI whereas the ELM neoepitope was related to pulmonary diseases. These results indicate that elastin neoepitopes generated by the same proteases but at different amino acid sites provide different tissue-related information depending on the disease in question.
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Authors | Helene Skjøt-Arkil, Rikke E Clausen, Lars M Rasmussen, Wanchun Wang, Yaguo Wang, Qinlong Zheng, Hans Mickley, Lotte Saaby, Axel C P Diederichsen, Jess Lambrechtsen, Fernando J Martinez, Cory M Hogaboam, Meilan Han, Martin R Larsen, Arkadiusz Nawrocki, Ben Vainer, Dorrit Krustrup, Marina Bjørling-Poulsen, Morten A Karsdal, Diana J Leeming |
Journal | PloS one
(PLoS One)
Vol. 8
Issue 6
Pg. e60936
( 2013)
ISSN: 1932-6203 [Electronic] United States |
PMID | 23805173
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Monoclonal, Murine-Derived
- Epitopes
- Elastin
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Topics |
- Antibodies, Monoclonal, Murine-Derived
(chemistry)
- Elastin
(blood)
- Enzyme-Linked Immunosorbent Assay
(methods)
- Epitopes
(blood)
- Female
- Humans
- Idiopathic Pulmonary Fibrosis
(blood)
- Male
- Myocardial Infarction
(blood)
- Plaque, Atherosclerotic
(blood)
- Proteolysis
- Pulmonary Disease, Chronic Obstructive
(blood)
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